THE KINETICS OF CHONDROITIN 4-SULFATE RELEASE FROM STIMULATED PLATELETS AND ITS RELATION TO THROMBOXANE A(2) FORMATION AND GRANULE SECRETION

dc.contributor.authorDonato, J. L.
dc.contributor.authorNogueira, Marie Doki [UNIFESP]
dc.contributor.authorMarcondes, S.
dc.contributor.authorAntunes, E.
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorDietrich, Carl Peter [UNIFESP]
dc.contributor.authorDenucci, G.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T14:28:09Z
dc.date.available2018-06-15T14:28:09Z
dc.date.issued1994-09-01
dc.description.abstract1. In platelet rich plasma (PRP), chondroitin 4-sulfate release from platelets occurred after stimulation with ADP (5 mu M), collagen (5-10 mu g/ml), or adrenaline (10 mu M). Release started within 60 s and maximum release (0.7-2.0 mg/l) was reached within 180 s. TXA(2) formation and dense granule release reached a maximum within 90 s after stimulation.2. Using washed platelets (1.5 x 10(8) cells/ml), the platelet responses were faster. Release of chondroitin 4-sulfate and TXA(2) started within 20-30 s after thrombin addition (100 mU/ml). Maximum release was reached within 60 s in both cases. Dense granule release started in the first 5 s of stimulation (34.6 +/- 12.4%) reaching maximum secretion (74.4 +/- 8.7%) within 60 s.3. Our results demonstrate that maximal chondroitin 4-sulfate release occurs after the dense granule release reaction in both PRP and washed platelets. This observation suggests that chondroitin 4-sulfate is unlikely to be stored in the dense granules but may be stored in the alpha-granules.en
dc.description.affiliationUNIV CAMPINAS,FAC CIENCIAS MED,DEPT FARMACOL,BR-13081970 CAMPINAS,SP,BRAZIL
dc.description.affiliationESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04023900 SAO PAULO,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04023900 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent2163-2167
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 9, p. 2163-2167, 1994.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43075
dc.identifier.wosWOS:A1994PG03900009
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsAcesso restrito
dc.subjectCHONDROITIN 4-SULFATEen
dc.subjectTHROMBOXANE-A(2)en
dc.subject5-HT RELEASEen
dc.subjectPLATELET AGGREGATIONen
dc.titleTHE KINETICS OF CHONDROITIN 4-SULFATE RELEASE FROM STIMULATED PLATELETS AND ITS RELATION TO THROMBOXANE A(2) FORMATION AND GRANULE SECRETIONen
dc.typeArtigo
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