Extracellular matrix alterations in the Peyronie's disease

dc.citation.issue4]
dc.citation.volume8]
dc.contributor.authorWatanabe, Marcelo Silva
dc.contributor.authorTheodoro, Therese Rachel
dc.contributor.authorCoelho, Natalia Lima [UNIFESP]
dc.contributor.authorMendes, Aline [UNIFESP]
dc.contributor.authorPereira Leonel, Monica Luzia
dc.contributor.authorMader, Ana Maria
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorGlina, Sidney
dc.contributor.authorSilva Pinhal, Maria Aparecida [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-06-26T16:30:23Z
dc.date.available2020-06-26T16:30:23Z
dc.date.issued2017
dc.description.abstractPeyronie's disease is characterized by fibrous plaque formation of the tunica albuginea, causing penile deformity and fertility problems. The aim of the present study was to investigate alterations in the extracellular matrix in Peyronie's disease. The study used tissues collected by surgical procedure from individuals that presented a well-established disease, while control samples were obtained by biopsies of fresh cadavers. Immunohistochemistry analysis followed by digital quantification was performed to evaluate TGF-beta, heparanases and metalloproteinases (MMPs). The profile of sulfated glycosaminoglycans, chondroitin sulfate and dermatan sulfate was determined by agarose gel electrophoresis, while hyaluronic acid quantification was obtained by an ELISA-like assay. The expression of mRNA was investigated for syndecan-1 proteoglycan (Syn-1), interleukine-6 (IL-6), hyaluronic acid synthases, and hyaluronidases. Pathologic features showed decreased apoptosis and blood vessel number in Peyronie's tissues. TGF-beta and IL-6 were significantly enhanced in Peyronie's disease. There was an increased expression of heparanases, though no alteration was observed for MMPs. Hyaluronic acid as well as hyaluronic acid synthases, hyaluronidases, and dermatan sulfate were not changed, while the level of chondroitin sulfate was significantly (P = 0.008, Mann-Whitney test) increased in Peyronie's samples. Heparanases and sulfated glycosaminoglycans seem to be involved in extracellular matrix alterations in Peyronie's disease. (C) 2017 Production and hosting by Elsevier B.V. on behalf of Cairo University.en
dc.description.affiliationFac Med ABC, Dept Biochem, BR-09060650 Santo Andre, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Dept Biochem, BR-04044020 Sao Paulo, Brazil
dc.description.affiliationFac Med ABC, Dept Pathol, BR-09060650 Santo Andre, Brazil
dc.description.affiliationFac Med ABC, Dept Urol, BR-09060650 Santo Andre, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Dept Biochem, BR-04044020 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent455-461
dc.identifierhttp://dx.doi.org/10.1016/j.jare.2017.06.004]
dc.identifier.citationJournal Of Advanced Research. Amsterdam, v. 8, n. 4, p. 455-461, 2017.
dc.identifier.doi10.1016/j.jare.2017.06.004
dc.identifier.fileWOS000411189500018.pdf
dc.identifier.issn2090-1232
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53518
dc.identifier.wosWOS:000411189500018
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Advanced Research
dc.rightsACESSO ABERTO
dc.subjectPeyronieen
dc.subjectTGF-betaen
dc.subjectInterleukin-6en
dc.subjectGlycosaminoglycansen
dc.subjectHeparanaseen
dc.subjectChondroitin sulfateen
dc.subjectMetalloproteinasesen
dc.titleExtracellular matrix alterations in the Peyronie's diseaseen
dc.typeArtigo
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