Association between the seminal plasma proteome and sperm functional traits

Página do item simplificado
dc.citation.issue3
dc.citation.volume105
dc.contributor.authorIntasqui, Paula [UNIFESP]
dc.contributor.authorCamargo, Mariana [UNIFESP]
dc.contributor.authorAntoniassi, Mariana Pereira [UNIFESP]
dc.contributor.authorCedenho, Agnaldo Pereira [UNIFESP]
dc.contributor.authorCarvalho, Valdemir Melechco
dc.contributor.authorMorais Cardozo, Karina Helena
dc.contributor.authorZylbersztejn, Daniel Suslik [UNIFESP]
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-08-21T17:00:03Z
dc.date.available2020-08-21T17:00:03Z
dc.date.issued2016
dc.description.abstractObjective: To analyze the seminal plasma proteome and biological functions associated with sperm functional alterations. Design: Cross-sectional study. Setting: University andrology and research laboratories. Patient(s): A total of 156 normozoospermic men. Intervention(s): Sperm mitochondrial activity, acrosome integrity, and DNA fragmentation were evaluated in a semen aliquot. Remaining semen was centrifuged, and seminal plasma was utilized for proteomic analysis (liquid chromatography-tandem mass spectrometry). Patients were divided into percentiles (15%) to form the following groups: substudy 1, high (control, n = 26) and low (study, n = 23) sperm mitochondrial activity; substudy 2, high (control, n = 23) and low (study, n = 22) sperm acrosome integrity; and substudy 3, low (control, n = 22) and high (study, n = 22) sperm DNA fragmentation. Groups were compared using univariate and multivariate analyses. Differentially expressed proteins were used for functional enrichment analysis. Main Outcome Measure(s): Seminal plasma proteome and postgenomic pathways are associated with several sperm functional traits. Result(s): In total, 506, 493, and 464 proteins were observed in substudies 1, 2, and 3, respectively. Enriched functions in substudy 1 were intramolecular oxidoreductase activity, aminoglycans catabolism, endopeptidases inhibition, lysosomes, and acute-phase response (study group). In substudy 2, main enriched functions were phospholipase inhibition, arachidonic acid metabolism, exocytosis, regulation of acute inflammation, response to hydrogen peroxide, and lysosomal transport (study group). In substudy 3, enriched functions were prostaglandin biosynthesis and fatty acid binding (study group). We proposed eight, six, and eight seminal biomarkers for substudies 1, 2, and 3, respectively. Conclusion(s): Seminal plasma proteome reflects sperm mitochondrial activity reduction, acrosome damage, and DNA fragmentation, with several postgenomic functions related to these alterations. (C) 2016 by American Society for Reproductive Medicine.en
dc.description.affiliationUniv Fed Sao Paulo, Human Reprod Sect, Div Urol, Dept Surg, R Embau 231, BR-04039060 Sao Paulo, SP, Brazil
dc.description.affiliationFleury Grp, Sao Paulo, Brazil
dc.description.affiliationSao Paulo Hosp, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Human Reprod Sect, Div Urol, Dept Surg, R Embau 231, BR-04039060 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFleury projects
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq)
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipIDCNPq: 472941/2012-7
dc.description.sponsorshipIDFAPESP: 2012/1463107
dc.format.extent617-628
dc.identifierhttp://dx.doi.org/10.1016/j.fertnstert.2015.11.005
dc.identifier.citationFertility And Sterility. New York, v. 105, n. 3, p. 617-628, 2016.
dc.identifier.doi10.1016/j.fertnstert.2015.11.005
dc.identifier.issn0015-0282
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57855
dc.identifier.wosWOS:000373406300014
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofFertility And Sterility
dc.rightsAcesso restrito
dc.subjectAcrosomeen
dc.subjectDNA damageen
dc.subjectmitochondriaen
dc.subjectproteomicsen
dc.subjectsemenen
dc.titleAssociation between the seminal plasma proteome and sperm functional traitsen
dc.typeArtigo
Arquivos
Coleções
EPM - Artigos