The effect of NaCl on the adsorption of human IgG onto CM-Asp-PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions

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dc.contributor.authorPavan, Gisele Luiza
dc.contributor.authorLazzarotto Bresolin, Igor Tadeu [UNIFESP]
dc.contributor.authorBorsoi-Ribeiro, Mariana
dc.contributor.authorVijayalakshmi, Mookambesvaran
dc.contributor.authorAlves Bueno, Sonia Maria
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionVIT Univ
dc.date.accessioned2016-01-24T14:37:39Z
dc.date.available2016-01-24T14:37:39Z
dc.date.issued2014-08-01
dc.description.abstractOver the past decade, immobilized metal-affinity adsorbents have attracted increasing interest for purification of natural and recombinant immunoglobulin G (IgG). in this work, nickel and cobalt metal ions complexed with CM-Asp (carboxymethylaspartate) immobilized on poly(ethylenevinyl alcohol) (PEVA) hollow fiber membranes were evaluated for purification of human IgG from serum. the buffer system and NaCl had important effects on human serum protein adsorption in both adsorbents. Efficient purification of IgG was accomplished in sodium phosphate buffer without NaCl at pH 7.0. Under this condition, the electrostatic interactions are important for adsorption. the Ni(II)-CM-Asp-PEVA had a protein adsorption capacity of 17.5 mg of IgG mL(-1) fiber when human serum diluted was loaded in crossflow filtration mode and the eluted IgG had a purity of 82.6 % (based on total protein and IgG, IgM, HSA, and Trf nephelometric analysis). Fitting the experimental IgG adsorption data to the Langmuir and Langmuir-Freundlich models showed that Ni(II)-CM-Asp and Co(II)-CM-Asp had Langmuirean and non-Langmuirean behavior, respectively, with positive cooperativity for IgG-Co(II)-CM-Asp binding, probably due to multipoint interactions (n = 2.12 +/- A 0.31). Thus, these membranes can be considered as alternative adsorbents for the purification or depletion of IgG from human serum.en
dc.description.affiliationUniv Estadual Campinas, Sch Chem Engn, UNICAMP, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Exact & Earth Sci, UNIFESP, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationVIT Univ, Ctr Bioseparat Technol, Vellore 632014, Tamil Nadu, India
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Exact & Earth Sci, UNIFESP, BR-09972270 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent677-688
dc.identifierhttp://dx.doi.org/10.1007/s10450-014-9612-1
dc.identifier.citationAdsorption-Journal of the International Adsorption Society. Dordrecht: Springer, v. 20, n. 5-6, p. 677-688, 2014.
dc.identifier.doi10.1007/s10450-014-9612-1
dc.identifier.issn0929-5607
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38032
dc.identifier.wosWOS:000339103300003
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofAdsorption-Journal of the International Adsorption Society
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectHuman IgGen
dc.subjectPurificationen
dc.subjectAffinity membraneen
dc.subjectIMACen
dc.subjectCM-Aspen
dc.titleThe effect of NaCl on the adsorption of human IgG onto CM-Asp-PEVA hollow fiber membrane-immobilized nickel and cobalt metal ionsen
dc.typeinfo:eu-repo/semantics/article
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