Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil

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dc.citation.issue4
dc.citation.volumev. 67
dc.contributor.authorHerkert, Patricia Fernanda
dc.contributor.authorMeis, Jacques F.
dc.contributor.authorSalvador, Gabriel Lucca de Oliveira
dc.contributor.authorGomes, Renata Rodrigues
dc.contributor.authorVicente, Vania Aparecida
dc.contributor.authorMuro, Marisol Dominguez
dc.contributor.authorPinheiro, Rosangela Lameira
dc.contributor.authorColombo, Arnaldo Lopes [UNIFESP]
dc.contributor.authorSchwarzbold, Alexandre Vargas
dc.contributor.authorOliveira, Carla Sakuma de
dc.contributor.authorFerreira, Marcelo Simao
dc.contributor.authorQueiroz-Telles, Flavio
dc.contributor.authorHagen, Ferry
dc.coverageLondon
dc.date.accessioned2020-07-20T16:31:07Z
dc.date.available2020-07-20T16:31:07Z
dc.date.issued2018
dc.description.abstractPurpose. Cryptococcosis is acquired from the environment by the inhalation of Cryptococcus cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a Cryptococcus neoformans species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of C. neoformans sensu lato isolates from southern Brazil. Methodology. We studied 219 C. neoformans sensu lato isolates with mating-and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing. Results/Key findings. Among the isolates, 136 (69 %) were from HIV-positive patients. Only C. neoformans mating-type a and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (n=172en
dc.description.abstract78.5 %), AFLP1A/VNII (n=19en
dc.description.abstract8.7 %), AFLP1B/VNII (n=4en
dc.description.abstract1.8 %) and a new AFLP pattern AFLP1C (n=23en
dc.description.abstract10.5 %). All isolates were susceptible to tested antifungals and no correlation between antifungal susceptibility and genotypes was observed. Through microsatellite analysis, most isolates clustered in a major microsatellite complex and Simpson's diversity index of this population was D=0.9856. Conclusion. The majority of C. neoformans sensu stricto infections occurred in HIV-positive patients. C. neoformans AFLP1/VNI was the most frequent genotype and all antifungal drugs had high in vitro activity against this species. Microsatellite analyses showed a high genetic diversity within the regional C. neoformans sensu stricto population, and correlation between environmental and clinical isolates, as well as a temporal and geographic relationship.en
dc.description.affiliationUniv Fed Parana, Dept Basic Pathol, Postgrad Program Microbiol Parasitol & Pathol Bio, Curitiba, Parana, Brazil
dc.description.affiliationCanisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands
dc.description.affiliationCAPES Fdn, Minist Educ Brazil, Brasilia, DF, Brazil
dc.description.affiliationCtr Expertise Mycol, Radboudumc CWZ, Nijmegen, Netherlands
dc.description.affiliationUniv Fed Parana, Dept Internal Med, Curitiba, Parana, Brazil
dc.description.affiliationState Univ Parana, Dept Biol Sci, Campus Paranagua, Paranagua, PR, Brazil
dc.description.affiliationUniv Fed Parana, Hosp Clin, Lab Mycol, Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Hosp Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Santa Maria, Hosp Univ, Santa Maria, RS, Brazil
dc.description.affiliationUniv Estadual Oeste Parana, Hosp Univ Oeste Parana, Cascavel, Brazil
dc.description.affiliationUniv Fed Uberlandia, Uberlandia, MG, Brazil
dc.description.affiliationUniv Fed Parana, Hosp Clin, Comunnitarian Hlth Dept, Curitiba, Parana, Brazil
dc.description.affiliationWesterdijk Fungal Biodivers Inst, Dept Med Mycol, Utrecht, Netherlands
dc.description.affiliationUnifespUniv Fed Sao Paulo, Hosp Sao Paulo, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil
dc.format.extent560-569
dc.identifierhttp://dx.doi.org/10.1099/jmm.0.000698
dc.identifier.citationJournal Of Medical Microbiology. London, v. 67, n. 4, p. 560-569, 2018.
dc.identifier.doi10.1099/jmm.0.000698
dc.identifier.issn0022-2615
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55714
dc.identifier.wosWOS:000429658500014
dc.language.isoeng
dc.publisherMicrobiology Soc
dc.relation.ispartofJournal Of Medical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCryptococcosisen
dc.subjectCryptococcus neoformansen
dc.subjectamplified fragment length polymorphismen
dc.subjectmicrosatellite typingen
dc.subjectmating-typeen
dc.subjectantifungal susceptibilityen
dc.titleMolecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazilen
dc.typeinfo:eu-repo/semantics/article
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