Candida albicans: The Ability to Invade Epithelial Cells and Survive under Oxidative Stress Is Unlinked to Hyphal Length

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dc.citation.volume8]
dc.contributor.authorMaza, Paloma K. [UNIFESP]
dc.contributor.authorBonfim-Melo, Alexis [UNIFESP]
dc.contributor.authorPadovan, Ana C. B. [UNIFESP]
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorOrikaza, Cristina M. [UNIFESP]
dc.contributor.authorDamas Ramos, Lilian M.
dc.contributor.authorMoura, Tauany R.
dc.contributor.authorSoriani, Frederico M.
dc.contributor.authorAlmeida, Ricardo S.
dc.contributor.authorSuzuki, Erika [UNIFESP]
dc.contributor.authorBahia, Diana [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-06-26T16:30:15Z
dc.date.available2020-06-26T16:30:15Z
dc.date.issued2017
dc.description.abstractIn its hyphal form, Candida albicans invades epithelial and endothelial cells by two distinct mechanisms: active penetration and induced endocytosis. The latter is dependent on a reorganization of the host cytoskeleton (actin/cortactin recruitment), whilst active penetration does not rely on the host's cellular machinery. The first obstacle for the fungus to reach deep tissues is the epithelial barrier and this interaction is crucial for commensal growth, fungal pathogenicity and host defense. This study aimed to characterize in vitro epithelial HeLa cell invasion by four different isolates of C. albicans with distinct clinical backgrounds, including a C. albicans SC5314 reference strain. All isolates invaded HeLa cells, recruited actin and cortactin, and induced the phosphorylation of both Src-family kinases (SFK) and cortactin. Curiously, L3881 isolated from blood culture of a patient exhibited the highest resistance to oxidative stress, although this isolate showed reduced hyphal length and displayed the lowest cell damage and invasion rates. Collectively, these data suggest that the ability of C. albicans to invade HeLa cells, and to reach and adapt to the host's blood, including resistance to oxidative stress, may be independent of hyphal length.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Disciplina Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Med, Lab Especial Micol,Disciplina Infectol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Alfenas, Dept Microbiol & Imunol, Alfenas, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Microbiol, Lab Micol Med & Microbiol Bucal, Londrina, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Disciplina Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Med, Lab Especial Micol,Disciplina Infectol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq
dc.description.sponsorshipFAPESP
dc.description.sponsorshipFAPEMIG
dc.description.sponsorshipIDCNPq: 477867/2010-3
dc.description.sponsorshipIDFAPESP: 2011/22773-6
dc.description.sponsorshipIDFAPESP: 2015/25652-6
dc.description.sponsorshipIDFAPEMIG: APQ-01674-14
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2017.01235]
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.01235
dc.identifier.fileWOS000406229100001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53444
dc.identifier.wosWOS:000406229100001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCandida albicansen
dc.subjecthyphaeen
dc.subjectHeLa cellsen
dc.subjectactinen
dc.subjectcortactinen
dc.subjectcell signalingen
dc.titleCandida albicans: The Ability to Invade Epithelial Cells and Survive under Oxidative Stress Is Unlinked to Hyphal Lengthen
dc.typeinfo:eu-repo/semantics/article
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