Preparation, Characterization, Cytotoxicity, and Genotoxicity Evaluations of Thiolated- and S-Nitrosated Superparamagnetic Iron Oxide Nanoparticles: Implications for Cancer Treatment

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dc.contributor.authorSeabra, Amedea Barozzi [UNIFESP]
dc.contributor.authorPasquoto, Tatiane
dc.contributor.authorFerrarini, Ana Carolina Ferraz [UNIFESP]
dc.contributor.authorSantos, Marconi da Cruz [UNIFESP]
dc.contributor.authorHaddad, Paula S. [UNIFESP]
dc.contributor.authorLima, Renata de
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Sorocaba
dc.date.accessioned2016-01-24T14:37:28Z
dc.date.available2016-01-24T14:37:28Z
dc.date.issued2014-07-01
dc.description.abstractIron oxide magnetic nanoparticles have been proposed for an increasing number of biomedical applications, such as drug delivery. To this end, toxicological studies of their potent effects in biological media must be better evaluated. the aim of this study was to synthesize, characterize, and examine the potential in vitro cytotoxicity and genotoxicity of thiolated (SH) and S-nitrosated (S-NO) iron oxide superparamagnetic nanoparticles toward healthy and cancer cell lines. Fe3O4 nanoparticles were synthesized by coprecipitation techniques and coated with small thiol-containing molecules, such as mercaptosuccinic acid (MSA) or meso-2,3-dimercaptosuccinic acid (DMSA). the physical chemical, morphological, and magnetic properties of thiol-coating Fe3O4 nanoparticles were characterized by different techniques. the thiol groups on the surface of the nanoparticles were nitrosated, leading to the formation of S-nitroso-MSA- or S-nitroso-DMSA-Fe3O4 nanoparticles. the cytotoxicity and genotoxicity of thiolated and S-nitrosated nanoparticles were more deeply evaluated in healthy (3T3, human lymphocytes cells, and chinese hamster ovary cells) and cancer cell lines (MCF-7). the results demonstrated that thiol-coating iron oxide magnetic nanoparticles have few toxic effects in cells, whereas S-nitrosated-coated particles did cause toxic effects. Moreover, due to the superaramagnetic behavior of S-nitroso-Fe3O4 nanopartides, those particles can be guided to the target site upon the application of an external magnetic field, leading to local toxic effects in the tumor cells. Taken together, the results suggest the promise of S-nitroso-mgnetic nanopartides in cancer treatment.en
dc.description.affiliationUniversidade Federal de São Paulo, Exact & Earth Sci Dept, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, UFSCar, BR-18052780 São Paulo, Brazil
dc.description.affiliationUniv Sorocaba, Dept Biotechnol, BR-18023000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Exact & Earth Sci Dept, BR-09913030 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2012/17053-7
dc.description.sponsorshipIDFAPESP: 2011/10125-0
dc.format.extent1207-1218
dc.identifierhttp://dx.doi.org/10.1021/tx500113u
dc.identifier.citationChemical Research in Toxicology. Washington: Amer Chemical Soc, v. 27, n. 7, p. 1207-1218, 2014.
dc.identifier.doi10.1021/tx500113u
dc.identifier.issn0893-228X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37888
dc.identifier.wosWOS:000339462700013
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofChemical Research in Toxicology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePreparation, Characterization, Cytotoxicity, and Genotoxicity Evaluations of Thiolated- and S-Nitrosated Superparamagnetic Iron Oxide Nanoparticles: Implications for Cancer Treatmenten
dc.typeinfo:eu-repo/semantics/article
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