Leptin fragments induce Fos immunoreactivity in rat hypothalamus

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dc.contributor.authorOliveira, V. X.
dc.contributor.authorFazio, M. A.
dc.contributor.authorMiranda, MTM
dc.contributor.authorSilva, J. M. da
dc.contributor.authorBittencourt, J. C.
dc.contributor.authorElias, C. F.
dc.contributor.authorMiranda, A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:37:48Z
dc.date.available2016-01-24T12:37:48Z
dc.date.issued2005-04-15
dc.description.abstractLeptin presents an important role in energy balance and neuroendocrine control in mammals. in an attempt to identify regions of the leptin molecule responsible for its bioactivity, we have synthesized six peptides based on the protein three-dimensional structure. Fragments were synthesized by the solid-phase methodology, purified by reverse-phase high-performance liquid chromatography (RP-HPLC), and characterized by liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS). They were injected intravenously and their ability to induce Fos immunoreactivity (Fos-ir) in rat hypothalamus was compared with that of the recombinant human leptin and saline. Fragment Ac-[Ser(117)]Lep(116-140)-NH2 (V) induced Fos-ir in hypothalamic nuclei that express leptin receptor long form. No similar ability was observed for the other five fragments. To investigate whether Fos-ir was induced in the same neuronal group activated by leptin, we proceeded with a dual-label immunohistochemistry for cocaine- and amphetamine-regulated transcript (CART), a neuropeptide related to leptin action in rat hypothalamus. We found that Ac-[Ser(117)]Lep(116-140)-NH2 (V) differentially activates CART neurons through the rostrocaudal extension of the arcuate nucleus. These results suggest that this fragment acts in the same group of neurons that mediate leptin response. This approach may offer the basis for the development of leptin-related compounds, having potential application in human or veterinary medicine. (C) 2004 Published by Elsevier B.V.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Chem, Dept Biochem, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Anat, Lab Chem Neuroanat, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent123-132
dc.identifierhttp://dx.doi.org/10.1016/j.regpep.2004.11.001
dc.identifier.citationRegulatory Peptides. Amsterdam: Elsevier B.V., v. 127, n. 1-3, p. 123-132, 2005.
dc.identifier.doi10.1016/j.regpep.2004.11.001
dc.identifier.issn0167-0115
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28253
dc.identifier.wosWOS:000227023900015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRegulatory Peptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectsynthetic peptidesen
dc.subjectobesityen
dc.subjectCARTen
dc.subjectenergy balanceen
dc.titleLeptin fragments induce Fos immunoreactivity in rat hypothalamusen
dc.typeinfo:eu-repo/semantics/article
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