Anti-secretory, anti-inflammatory and anti-Helicobacter pylori activities of several fractions isolated from Piper carpunya Ruiz & Pav.

dc.contributor.authorQuilez, A.
dc.contributor.authorBerenguer, B.
dc.contributor.authorGilardoni, G.
dc.contributor.authorSouccar, C. [UNIFESP]
dc.contributor.authorMendonca, S. de
dc.contributor.authorOliveira, L. F. S.
dc.contributor.authorMartin-Calero, M. J.
dc.contributor.authorVidari, G.
dc.contributor.institutionUniv Seville
dc.contributor.institutionUniv Pavia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Bandeirante São Paulo
dc.description.abstractEthnopharmacological relevance: the leaves of Piper carpunya Ruiz & Pay. (syn Piper lenticellosum C.D.C.) (Piperaceae), are widely used in folk medicine in tropical and subtropical countries of South America as an anti-inflammatory, anti-ulcer, anti-diarrheal and anti-parasitical remedy as well as an ailment for skin irritations.Aims of the study: To study the anti-inflammatory, anti-secretory and anti-Helicobacter pylori activities of different fractions isolated from an ethanolic extract of the leaves of Piper carpunya, in order to provide evidence for the use of this plant as an anti-ulcer remedy. Moreover, to isolate the main compounds of the extract and relate their biological activity to the experimental results obtained with the fractions.Materials and methods: Sixteen fractions were obtained from the ethanolic extract (FI-XVI) and 16 pure compounds were isolated and identified from these fractions. We studied the effects of the fractions (0.1-400 mu g/mL) on the release of myeloperoxidase (MPO) enzyme from rat peritoneal leukocytes, on rabbit gastric microsomal H(+), K(+)-ATPase activity and anti-Helicobacter pylori anti-microbial activity using the microdilution method (MM). the main compounds contained in the fractions were isolated and identified by (1)H- and (13)C NMR spectra analysis and comparison with the literature data.Results: Eight fractions showed inhibition of MPO enzyme (FI-IV, X, XII, XIV and XV). the highest inhibition was observed with F XIV (50 mu g/mL, 60.9%, p < 0.001). F X and XII were the most active ones, inhibiting the gastric H(+), K(+)-ATPase activity with IC(50) values equal to 22.3 mu g/mL and 28.1 mu g/mL, respectively. All fractions, except F XV, presented detectable anti-Helicobacter pylori activity, with a diameter of inhibition zones ranging from 11 mm up to 50 mm. the best anti-Helicobacter pylori activity was obtained with F III and V. Both fractions killed Helicobacter pylori with lowest concentration values, about 6.25 mu g/mL. Sixteen pure compounds were isolated, five of them are flavonoids that possess strong anti-oxidant and free radical scavenging activity, e.g. vitexin, isovitexin, and rhamnopyranosylvitexin. Terpenoids like sitosterol, stigmasterol and phytol, which have shown gastroprotective activity, and dihydrochalcones, like asebogenin, with anti-bacterial activity, were also isolated. Furthermore, the rare neolignan 1, that is a DNA polymerase 3 lyase inhibitor, and (6S, 9S)-roseoside, that shows strong anti-bacterial activity, were isolated, for the first time, from the genus Piper.Conclusions: We suggest that the flavonoids isolated from F I and II (vitexin, isovitexin, rhamnopyranosylvitexin and isoembigenin) contribute to the anti-MPO activity, as well as to their anti-Helicobacter pylori activity. These flavonoids may also be responsible for the important inhibition of H(+), K(+)-APase activity. Also the phytosterols and phytol obtained from F XIV and XV could be involved in these gastroprotective activities. These results encourage us to continue phytochemical studies on these fractions in order to obtain full scientific validation for this species. (C) 2010 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniv Seville, Dept Pharmacol, Fac Pharm, E-41012 Seville, Spain
dc.description.affiliationUniv Pavia, Dept Organ Chem, I-27100 Pavia, Italy
dc.description.affiliationUniv Pavia, Ctr Ethnobiopharm CISTRE, I-27100 Pavia, Italy
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUniv Bandeirante São Paulo, Dept Pharmacol, UNIBAN, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMIUR of Italy
dc.description.sponsorshipIDMIUR of Italy: 2007
dc.identifier.citationJournal of Ethnopharmacology. Clare: Elsevier B.V., v. 128, n. 3, p. 583-589, 2010.
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Ethnopharmacology
dc.rightsAcesso restrito
dc.subjectPiper carpunyaen
dc.subjectPrenylated benzoic acid derivativesen
dc.subjectH(+), K(+)-ATPase activityen
dc.subjectAnti-inflammatory activityen
dc.subjectAnti-Helicobacter pylori activityen
dc.titleAnti-secretory, anti-inflammatory and anti-Helicobacter pylori activities of several fractions isolated from Piper carpunya Ruiz & Pav.en