Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi

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dc.contributor.authorBarros, Roberto Rudge de Moraes [UNIFESP]
dc.contributor.authorMarini, Marjorie Mendes [UNIFESP]
dc.contributor.authorAntonio, Cristiane Regina [UNIFESP]
dc.contributor.authorCortez, Danielle Rodrigues [UNIFESP]
dc.contributor.authorMiyake, Andrea Midory [UNIFESP]
dc.contributor.authorLima, Fabio Mitsuo [UNIFESP]
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorBartholomeu, Daniella Castanheira
dc.contributor.authorChiurillo, Miguel Angel
dc.contributor.authorRamirez, José Luis
dc.contributor.authorSilveira, Jose Franco da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFIOCRUZ MG
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUCLA
dc.contributor.institutionFdn Inst Estudios Avanzados IDEA
dc.date.accessioned2016-01-24T14:27:21Z
dc.date.available2016-01-24T14:27:21Z
dc.date.issued2012-06-08
dc.description.abstractBackground: the subtelomeres of many protozoa are highly enriched in genes with roles in niche adaptation. T. cruzi trypomastigotes express surface proteins from Trans-Sialidase (TS) and Dispersed Gene Family-1 (DGF-1) superfamilies which are implicated in host cell invasion. Single populations of T. cruzi may express different antigenic forms of TSs. Analysis of TS genes located at the telomeres suggests that chromosome ends could have been the sites where new TS variants were generated. the aim of this study is to characterize telomeric and subtelomeric regions of T. cruzi available in TriTrypDB and connect the sequences of telomeres to T. cruzi working draft sequence.Results: We first identified contigs carrying the telomeric repeat (TTAGGG). of 49 contigs identified, 45 have telomeric repeats at one end, whereas in four contigs the repeats are located internally. All contigs display a conserved telomeric junction sequence adjacent to the hexamer repeats which represents a signature of T. cruzi chromosome ends. We found that 40 telomeric contigs are located on T. cruzi chromosome-sized scaffolds. in addition, we were able to map several telomeric ends to the chromosomal bands separated by pulsed-field gel electrophoresis. the subtelomeric sequence structure varies widely, mainly as a result of large differences in the relative abundance and organization of genes encoding surface proteins (TS and DGF-1), retrotransposon hot spot genes (RHS), retrotransposon elements, RNA-helicase and N-acetyltransferase genes. While the subtelomeric regions are enriched in pseudogenes, they also contain complete gene sequences matching both known and unknown expressed genes, indicating that these regions do not consist of nonfunctional DNA but are instead functional parts of the expressed genome. the size of the subtelomeric regions varies from 5 to 182 kb; the smaller of these regions could have been generated by a recent chromosome breakage and telomere healing event.Conclusions: the lack of synteny in the subtelomeric regions suggests that genes located in these regions are subject to recombination, which increases their variability, even among homologous chromosomes. the presence of typical subtelomeric genes can increase the chance of homologous recombination mechanisms or microhomology-mediated end joining, which may use these regions for the pairing and recombination of free ends.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationFIOCRUZ MG, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, ICB, Dept Parasitol, Belo Horizonte, MG, Brazil
dc.description.affiliationUCLA, Barquisimeto, Venezuela
dc.description.affiliationFdn Inst Estudios Avanzados IDEA, Caracas, Venezuela
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent16
dc.identifierhttps://dx.doi.org/10.1186/1471-2164-13-229
dc.identifier.citationBmc Genomics. London: Biomed Central Ltd, v. 13, 16 p., 2012.
dc.identifier.doi10.1186/1471-2164-13-229
dc.identifier.fileWOS000307450600001.pdf
dc.identifier.issn1471-2164
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34990
dc.identifier.wosWOS:000307450600001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Genomics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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