Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis

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dc.contributor.authorMarques, Mariangela Esther Alencar
dc.contributor.authorSoares, Angela Maria Victoriano de Campos
dc.contributor.authorFranco, Marcello
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.authorMarques, Silvio Alencar
dc.contributor.authorMendes, Rinaldo Poncio
dc.contributor.authorSouza, Lenice do Rosário de
dc.contributor.authorCoelho, Kunie Iabuki Rabello
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T18:07:24Z
dc.date.available2018-06-15T18:07:24Z
dc.date.issued1996-07-01
dc.description.abstractThe exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.en
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTUCATU,DEPT PATOL,BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOCIENCIAS,DIV IMUNOL,BOTUCATU,SP,BRAZIL
dc.description.affiliationESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT DERMATOL,BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT MOLESTIAS INFECC,BOTUCATU,SP,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent265-272
dc.identifierhttps://dx.doi.org/10.1080/02681219680000451
dc.identifier.citationJournal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996.
dc.identifier.doi10.1080/02681219680000451
dc.identifier.issn0268-1218
dc.identifier.urihttps://repositorio.unifesp.br/11600/44493
dc.identifier.wosWOS:A1996VC30200006
dc.language.isoeng
dc.publisherBlackwell Science Ltd
dc.relation.ispartofJournal Of Medical And Veterinary Mycology
dc.rightsAcesso aberto
dc.subjectParacoccidioides brasiliensisen
dc.subjectParacoccidioidinen
dc.subjectParacoccidioidomycosisen
dc.titleEvaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosisen
dc.typeArtigo
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