Serotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattle

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dc.citation.issue4
dc.citation.volume121
dc.contributor.authorGonzalez, Alice Gonçalves Martins
dc.contributor.authorCerqueira, Aloysio de Mello Figueiredo
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorCoutinho, Cesar Augusto de Souza
dc.contributor.authorLiberal, Maira Halfen Teixeira
dc.contributor.authorSouza, Rossiane de Moura
dc.contributor.authorAndrade, João Ramos da Costa
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageHoboken
dc.date.accessioned2020-07-31T12:47:38Z
dc.date.available2020-07-31T12:47:38Z
dc.date.issued2016
dc.description.abstractAimThe occurrence of virulence markers, serotypes and invasive ability were investigated in Shiga toxin-producing Escherichia coli (STEC) isolated from faecal samples of healthy dairy cattle at Rio de Janeiro State, Brazil. Methods and ResultsFrom 1562 stx-positive faecal samples, 105 STEC strains were isolated by immuno-magnetic separation (IMS) or plating onto MacConkey agar (MC) followed by colony hybridisation. Fifty (476%) strains belonged to nine serotypes (O8:H19, O22:H8, O22:H16, O74:H42, O113:H21, O141:H21, O157:H7, O171:H2 and ONT:H21). The prevalent serotypes were O157:H7 (124%), O113:H21 (67%) and O8:H19 (57%). Virulence genes were identified by polymerase chain reaction (PCR). E-hlyA (771%) was the more prevalent virulence marker, followed by espP (648%), saa (39%), eae (248%) and astA (219%). All O157:H7 strains carried the (gamma) variant of the locus of enterocyte effacement (LEE) genes and the stx2c gene, while the stx1/stx2 genotype prevailed among the eae-negative strains. None of the eae-positive STEC produced the localized adherence (LA) phenotype in HEp-2 or Caco-2 cells. However, intimate attachment (judged by the fluorescent actin staining test) was detected in some eae-positive strains, both in HEp-2 (231%) and in Caco-2 cells (115%). Most strains (875%) showed peripheral association' (PA) adherence phenotype to undifferentiated Caco-2 cells. Twenty-five (926%) of 27 strains invaded Caco-2 cells. The highest average value of invasion (96%) was observed among the eae-negative bovine strains from serotypes described in human disease. ConclusionHealthy dairy cattle is a reservoir of STEC carrying virulence genes and properties associated with human disease. Significance and Impact of the StudyAlthough reports of human disease associated with STEC are scarce in Brazil, the colonization of the animal reservoir by potentially pathogenic strains offers a significant risk to our population.en
dc.description.affiliationUniv Fed Fluminense, Dept Bromatol, Niteroi, RJ, Brazil
dc.description.affiliationUniv Fed Fluminense, Dept Microbiol & Parasitol, Niteroi, RJ, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Dept Microbiol Imunol & Parasitol, Rio De Janeiro, Brazil
dc.description.affiliationPesagro Rio, Ctr Estadual Pesquisa Sanidade Anim, Niteroi, RJ, Brazil
dc.description.affiliationUnifespDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brasil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)/APQ1pt
dc.description.sponsorshipFOPESQ/UFF
dc.format.extent1130-1143
dc.identifierhttps://dx.doi.org/10.1111/jam.13230
dc.identifier.citationJournal Of Applied Microbiology. Hoboken, v. 121, n. 4, p. 1130-1143, 2016.
dc.identifier.doi10.1111/jam.13230
dc.identifier.issn1364-5072
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56954
dc.identifier.wosWOS:000383575800021
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Applied Microbiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCattleen
dc.subjectCell invasionen
dc.subjectEscherichia coli O157:H7en
dc.subjectSerotypesen
dc.subjectShiga toxin-producing Escherichia colien
dc.subjectVirulence markersen
dc.titleSerotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattleen
dc.typeinfo:eu-repo/semantics/article
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