Adhesion Molecules Affected by Treatment of Lung Cancer Cells with Epidermal Growth Factor

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Fonseca, Fernando L. A. [UNIFESP]
Azzalis, Ligia A. [UNIFESP]
Feder, David
Nogoceke, Everson
Junqueira, Virginia B. C. [UNIFESP]
Valenti, Vitor E.
Abreu, Luiz Carlos de
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Lung cancer is one of the leading causes of death in the world. Some tumor events are attributed to an important group of molecules (cadherins and integrins). We evaluated the interactions of cell adhesion molecules in cell lines from lung cancer. Two lung cancer cell lines were nonmetastatic (H358 and H441) and two were metastatic (H1299 and H292). All cell lines were treated with epidermal growth factor (EGF), and Western blot analysis was performed to assess the interactions between these proteins. the bronchoalveolar cells H358 showed the three analyzed proteins: E-cadherin, beta-catenin, and p120 catenin. the adenocarcinoma cells H441 did not present p120 catenin, and carcinoma cells did not show E-cadherin (H1299) or p120 catenin (H292). FAK (pTyr925) was dephosphorylated in adenocarcinoma cells H441, absent in carcinoma cells H1299, and upregulated in the other carcinoma cells H292. p130Cas showed no difference when the cell lines were treated with EGF for 30 min; it was absent in the metastatic carcinoma cells H1299. Paxillin was dephosphorylated in adenocarcinoma cells H441 and also absent in other metastatic carcinoma cells H292. Vinculin showed the same results, and talin was downregulated in adenocarcinoma cells H441 when the cells were treated with EGF. Rap1 was downregulated and PYK2 was upregulated in the same cell line. Our data help to comprehend the mechanism involved in cell migration to the blood and metastasis generation. in conclusion, the expression patterns of cell-cell adhesion were not affected by EGF treatment but it affected cell-extracellular matrix adhesion.
Lung. New York: Springer, v. 189, n. 5, p. 383-389, 2011.