Hpv genotyping and p16 expression in xingu indigenous park, brazil

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dc.contributor.authorFreitas, V. G. [UNIFESP]
dc.contributor.authorFocchi, G. R. [UNIFESP]
dc.contributor.authorPereira, E. R. [UNIFESP]
dc.contributor.authorLevi, J. E.
dc.contributor.authorSpeck, N. M. G. [UNIFESP]
dc.contributor.authorRibalta, J. C. [UNIFESP]
dc.date.accessioned2019-01-21T10:29:47Z
dc.date.available2019-01-21T10:29:47Z
dc.date.issued2016
dc.description.abstractThe association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec (R) Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 +/- 15.89 years) and parity ranged from 1-11 (5.92 +/- 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significanten
dc.description.abstractit was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotypeen
dc.description.abstracthowever, more studies are necessary to further our understanding of this restricted group.en
dc.description.affiliationNúcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationDepartamento de Patologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationDepartamento de Medicina Preventiva, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationInstituto de Medicina Tropical da Universidade de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifespNúcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifespDepartamento de Patologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifespDepartamento de Medicina Preventiva, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) grant
dc.format.extentUNSP 15036840
dc.identifierhttp://dx.doi.org/10.4238/gmr.15036840
dc.identifier.citationGenetics And Molecular Research. Ribeirao preto, v. 15, n. 3, p. UNSP 15036840, 2016.
dc.identifier.doi10.4238/gmr.15036840
dc.identifier.issn1676-5680
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49389
dc.identifier.wosWOS:000384881100006
dc.language.isoeng
dc.publisherUniv Fed Sao Carlos, Dept Engenharia Materials
dc.relation.ispartofGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectP16 Proteinen
dc.subjectP16 Expressionen
dc.subjectHuman Papillomavirusen
dc.subjectIndigenousen
dc.subjectXinguen
dc.subjectGenotypeCervical Intraepithelial Neoplasiaen
dc.subjectHuman-Papillomavirus Genotypesen
dc.subjectCanceren
dc.subjectWomenen
dc.subjectInfectionen
dc.subjectLesionsen
dc.subjectDiagnosisen
dc.subjectRisken
dc.subjectPrevalenceen
dc.subjectPreventionen
dc.titleHpv genotyping and p16 expression in xingu indigenous park, brazilen
dc.typeinfo:eu-repo/semantics/article
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