Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

dc.contributor.authorTorres, Alfredo G.
dc.contributor.authorBlanco, Miguel
dc.contributor.authorValenzuela, Patricio
dc.contributor.authorSlater, Terry M.
dc.contributor.authorPatel, Shilpa D.
dc.contributor.authorDahbi, Ghizlane
dc.contributor.authorLopez, Cecilia
dc.contributor.authorFernandez Barriga, Ximena
dc.contributor.authorBlanco, Jesus E.
dc.contributor.authorGomes, Tania A. T. [UNIFESP]
dc.contributor.authorVidal, Roberto
dc.contributor.authorBlanco, Jorge
dc.contributor.institutionUniv Texas Med Branch
dc.contributor.institutionUniv Santiago de Compostela
dc.contributor.institutionUniv Chile
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:58:34Z
dc.date.available2016-01-24T13:58:34Z
dc.date.issued2009-08-01
dc.description.abstractLpf (stands for long polar fimbriae) is one of the few adhesive factors of enterohemorrhagic Escherichia coli O157:H7 associated with colonization of the intestine. E. coli O157:H7 strains possess two lpf loci encoding highly regulated fimbrial structures. Database analysis of the genes encoding the major fimbrial subunits demonstrated that they are present in commensal as well as pathogenic (both intestinal and extraintestinal) E. coli strains and in Salmonella strains and that the lpfA1 and lpfA2 genes are highly prevalent among LEE (locus of enterocyte effacement)-positive E. coli strains associated with severe and/or epidemic disease. Further DNA sequence analysis of the lpfA1 and lpfA2 genes from different attaching-and-effacing E. coli strains has led us to the identification of several polymorphisms and the classification of the major fimbrial subunits into distinct variants. Using collections of pathogenic E. coli isolates from Europe and Latin America, we demonstrated that the different lpfA types are associated with the presence of specific intimin (eae) adhesin variants and, most importantly, that they are found in specific E. coli pathotypes. Our results showed that the use of these fimbrial genes as markers, in combination with the different intimin types, resulted in a specific test for the identification of E. coli O157:H7, distinguishing it from other pathogenic E. coli strains.en
dc.description.affiliationUniv Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA
dc.description.affiliationUniv Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA
dc.description.affiliationUniv Texas Med Branch, Sealy Ctr Vaccine Dev, Galveston, TX 77555 USA
dc.description.affiliationUniv Santiago de Compostela, Dept Microbiol & Parasitol, Lugo 27002, Spain
dc.description.affiliationUniv Chile, Fac Med, Inst Ciencias Biomed, Programa Microbiol, Santiago 7, Chile
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNIH
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cient fico e Tecnologico
dc.description.sponsorshipFONDECYT
dc.description.sponsorshipFondo de Investigacion Sanitaria
dc.description.sponsorshipXunta de Galicia
dc.description.sponsorshipComision Interministerial de Ciencia y Tecnologia
dc.description.sponsorshipIDNIH: AI079154-01A2
dc.description.sponsorshipIDFONDECYT: 1061088
dc.format.extent2442-2451
dc.identifierhttp://dx.doi.org/10.1128/JCM.00566-09
dc.identifier.citationJournal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 47, n. 8, p. 2442-2451, 2009.
dc.identifier.doi10.1128/JCM.00566-09
dc.identifier.fileWOS000268499600013.pdf
dc.identifier.issn0095-1137
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31696
dc.identifier.wosWOS:000268499600013
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal of Clinical Microbiology
dc.rightsAcesso aberto
dc.titleGenes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolatesen
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