Influence of TNF-alpha blockers on the oral prevalence of opportunistic microorganisms in ankylosing spondylitis patients

dc.contributor.authorPereira, D. F. A.
dc.contributor.authorPinheiro, Marcelo de Medeiros [UNIFESP]
dc.contributor.authorSilva, P. N. F.
dc.contributor.authorTeodoro, G. R.
dc.contributor.authorBrighenti, F. L.
dc.contributor.authorKoga-Ito, C. Y.
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T16:52:44Z
dc.date.available2018-06-15T16:52:44Z
dc.date.issued2012-09-01
dc.description.abstractObjectivesTo compare the oral prevalence and antimicrobial susceptibility of candida spp. staphylococci, enterobacteriaceae, and pseudomonas spp. from ankylosing spondylitis (AS) patients receiving conventional and anti-TNF-alpha therapy.MethodsThe study included 70 AS patients, diagnosed according to the modified New York criteria (1984). The volunteers were divided into 2 groups: a biological group (AS BioG) (n=35) (on anti-TNF-a therapy) and a conventional group (AS ConvG) (n=35). The control group (ContG) (n=70) was made up of healthy individuals matched for age, gender, and oral conditions. After clinical examination, oral rinse samples were collected and plated in specific culture media. The number of colony-forming units per milliliter (cfu/ml) was obtained, and isolates were identified using the API system. Antimicrobial susceptibility tests were performed according to the NCCLS guidelines. Prevalence and counts of microorganisms were statistically compared between the 3 groups, using the Mann-Whitney and Chi-square tests. Significance level was set at 5%.ResultsIn both the AS BioG and the AS ConvG, staphylococci counts were higher than that in the ContG (p<0.0001).Candida albicans and staphylococcus epidermidis were the most commonly found species in all the groups. Serratia marcescens and klebsiella oxytoca were more prevalent in the AS BioG and the AS ConvG, respectively. Two candida isolates (2.8%) from the AS BioG and 5 (10.8%) from the AS ConvG were resistant to amphotericin B and 5-fluorocytosine. A low percentage of staphylococci isolates was resistant to amoxicillin, ciprofloxacin, and doxycycline.ConclusionHigher counts of staphylococci were observed in both AS groups, regardless of the current therapy, age, sex, and oral conditions. Anti-TNF-alpha therapy could not be correlated with increased counts of microorganisms.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Sao Jose Dos Campos Dent Sch, BR-12245000 Sao Jose Dos Campos, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Rheumatol, EPM, Unifesp, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Rheumatol, EPM, Unifesp, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2008/57631-4
dc.description.sponsorshipIDFAPESP: 2008/56485-4
dc.format.extent679-685
dc.identifierhttp://www.clinexprheumatol.org/abstract.asp?a=5276
dc.identifier.citationClinical And Experimental Rheumatology. Pisa: Clinical & Exper Rheumatology, v. 30, n. 5, p. 679-685, 2012.
dc.identifier.issn0392-856X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43331
dc.identifier.wosWOS:000310828600006
dc.language.isoeng
dc.publisherClinical & Exper Rheumatology
dc.relation.ispartofClinical And Experimental Rheumatology
dc.rightsAcesso restrito
dc.subjectankylosing spondylitisen
dc.subjectinfectionen
dc.subjectsuper-infectionen
dc.subjectoral microbiologyen
dc.subjectTNF-alpha blockersen
dc.subjectcandidaen
dc.subjectstaphylococcusen
dc.titleInfluence of TNF-alpha blockers on the oral prevalence of opportunistic microorganisms in ankylosing spondylitis patientsen
dc.typeArtigo
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