Cleavage of human fibronectin and other basement membrane-associated proteins by a Cryptococcus neoformans serine proteinase

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dc.contributor.authorRodrigues, M. L.
dc.contributor.authorReis, FCG dos
dc.contributor.authorPuccia, R.
dc.contributor.authorTravassos, L. R.
dc.contributor.authorAlviano, C. S.
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:42Z
dc.date.available2016-01-24T12:33:42Z
dc.date.issued2003-02-01
dc.description.abstractThe interaction between the fungal pathogen Cryptococcus neoformans and human fibronectin (HFN) was examined in this study. Polypeptides from cryptococcal whole homogenates and cell wall with molecular masses of 25 and 35 kDa, respectively reacted with HFN. the relevance of the occurrence of these proteins in intact cells was uncertain, since yeast cells from different strains and serotypes of C neoformans did not significantly adhere to soluble or solid-phased HFN. in contrast, an exocellular proteolytic activity that cleaves HFN was suggested. Degradation of HFN by culture supernatant fluids was demonstrated by Western blotting using a monoclonal anti-HFN antibody. Several fragments of lower molecular weights were observed which reacted with the antibody. Proteolysis was mediated by a serine protease activity, since HFN cleavage was completely inhibited by phenylmethylsulfonyl fluoride (PMSF), aprotinin, and N-tosyl-L-phenylalanyl chloromethylketone (TPCK), but not by inhibitors of metalo, cysteine, or aspartyl proteases. Similar results were obtained when the fluorogenic peptide carbobenzoxy-phenylalanyl-arginyl-7-amido-4-methylcoumarin (CBZ-Phe-Arg-NHmet-C) was used as substrate. the cryptococcal supernatant also cleaved laminin and type IV collagen, as demonstrated by polyacrylamide gel electrophoresis with co-polymerized proteins. the hydrolysis of these proteins was mediated by a single cryptococcal protease with a molecular mass of 75 kDa. the cleavage of key host components of the basement membrane and extracellular matrix by C. neoformans may be a relevant factor in the process of fungal invasion. (C) 2003 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, Dept Microbiol Geral, BR-21941590 Rio de Janeiro, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941590 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-4023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-4023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent65-71
dc.identifierhttp://dx.doi.org/10.1016/S0882-4010(02)00195-X
dc.identifier.citationMicrobial Pathogenesis. London: Academic Press Ltd Elsevier B.V., v. 34, n. 2, p. 65-71, 2003.
dc.identifier.doi10.1016/S0882-4010(02)00195-X
dc.identifier.issn0882-4010
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27135
dc.identifier.wosWOS:000181757900002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobial Pathogenesis
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCryptococcus neoformansen
dc.subjectfibronectinen
dc.subjectbasement membraneen
dc.subjectfungal invasionen
dc.titleCleavage of human fibronectin and other basement membrane-associated proteins by a Cryptococcus neoformans serine proteinaseen
dc.typeinfo:eu-repo/semantics/article
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