Trypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activity

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dc.contributor.authorOliveira-, F. O. R.
dc.contributor.authorAlves, C. R.
dc.contributor.authorSilva, F. S.
dc.contributor.authorCortes, L. M. C.
dc.contributor.authorToma, L. [UNIFESP]
dc.contributor.authorBoucas, R. I. [UNIFESP]
dc.contributor.authorAguilar, T. [UNIFESP]
dc.contributor.authorNader, H. B. [UNIFESP]
dc.contributor.authorPereira, M. C. S.
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:14Z
dc.date.available2016-01-24T14:31:14Z
dc.date.issued2013-02-01
dc.description.abstractHeparin-binding proteins (HBPs) play a key role in Trypanosoma cruzi-host cell interactions. HBPs recognize heparan sulfate (HS) at the host cell surface and are able to induce the cytoadherence and invasion of this parasite. Herein, we analysed the biochemical properties of the HBPs and also evaluated the expression and subcellular localization of HBPs in T. cruzi trypomastigotes. A flow cytometry analysis revealed that HBPs are highly expressed at the surface of trypomastigotes, and their peculiar localization mainly at the flagellar membrane, which is known as an important signalling domain, may enhance their binding to HS and elicit the parasite invasion. the plasmon surface resonance results demonstrated the stability of HBPs and their affinity to HS and heparin. Additionally, gelatinolytic activities of 70 kDa, 65.8 kDa and 59 kDa HBPs over a broad pH range (5.5-8.0) were revealed using a zymography assay. These proteolytic activities were sensitive to serine proteinase inhibitors, such as aprotinin and phenylmethylsulfonyl fluoride, suggesting that HBPs have the properties of trypsin-like proteinases.en
dc.description.affiliationFiocruz MS, Inst Oswaldo Cruz, Lab Ultraestrutura Celular, BR-21045900 Rio de Janeiro, RJ, Brazil
dc.description.affiliationFiocruz MS, Inst Oswaldo Cruz, Lab Biol Mol & Doencas Endemicas, BR-21045900 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao Oswaldo Cruz (FIOCRUZ)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPrograma Estrategico de Apoio a Pesquisa em Saude
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 477292/2010-0
dc.description.sponsorshipIDCNPq: 509737/2010-2
dc.description.sponsorshipIDPrograma Estrategico de Apoio a Pesquisa em Saude: 403504/2008-2
dc.description.sponsorshipIDPrograma Estrategico de Apoio a Pesquisa em Saude: 300731/2010-8
dc.description.sponsorshipIDFAPERJ: E-26/110.322/2012
dc.description.sponsorshipIDFAPERJ: E-26/103.060/2008
dc.description.sponsorshipIDFAPERJ: E-26/110.257/2010
dc.description.sponsorshipIDCAPES: EDITAL - 11/2009
dc.description.sponsorshipIDFAPESP: 07/59801-1
dc.format.extent171-180
dc.identifierhttp://dx.doi.org/10.1017/S0031182012001448
dc.identifier.citationParasitology. New York: Cambridge Univ Press, v. 140, n. 2, p. 171-180, 2013.
dc.identifier.doi10.1017/S0031182012001448
dc.identifier.issn0031-1820
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35949
dc.identifier.wosWOS:000314294100003
dc.language.isoeng
dc.publisherCambridge Univ Press
dc.relation.ispartofParasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dc.subjectTrypanosoma cruzien
dc.subjectheparin-binding proteinen
dc.subjectplasmon surface resonanceen
dc.subjectserine proteinaseen
dc.titleTrypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activityen
dc.typeinfo:eu-repo/semantics/article
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