Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa

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dc.contributor.authorMelo, Gustavo Barreto de
dc.contributor.authorAggio, Fabio Bom
dc.contributor.authorHofling-Lima, Ana Luisa
dc.contributor.authord'Azevedo, Pedro Alves [UNIFESP]
dc.contributor.authorCampos Pignatari, Antonio Carlos
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:48:50Z
dc.date.available2016-01-24T13:48:50Z
dc.date.issued2007-07-01
dc.description.abstractPurpose To report the use of pulsed-field gel electrophoresis (PFGE) in the investigation of the origin of infectious keratitis.Methods A 19-year-old girl presented with infectious keratitis in the left eye. She was a soft contact lens wearer, and was noncompliant with the proper cleaning methods proposed by the manufacturer. Microbiological assessment was performed by means of culture and PFGE.Results the patient was treated successfully with topical cefalotin and gentamicin drops. Cultures were positive for Pseudomonas aeruginosa in the cornea and conjunctiva, and in the contact lens, its case and the cleaning solution. PFGE showed identical patterns of banding in each.Conclusion in this case of bacterial keratitis, PFGE proved to be very useful in identifying how the contamination occurred.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis, Special Lab Clin Microbiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis, Special Lab Clin Microbiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1053-1054
dc.identifierhttp://dx.doi.org/10.1007/s00417-006-0503-3
dc.identifier.citationGraefes Archive for Clinical and Experimental Ophthalmology. New York: Springer, v. 245, n. 7, p. 1053-1054, 2007.
dc.identifier.doi10.1007/s00417-006-0503-3
dc.identifier.issn0721-832X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29845
dc.identifier.wosWOS:000247503800021
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofGraefes Archive for Clinical and Experimental Ophthalmology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectpulsed-field gel electrophoresisen
dc.subjectbacterial keratitisen
dc.subjectPseudomonas aeruginosaen
dc.titlePulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosaen
dc.typeinfo:eu-repo/semantics/article
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