Grape juice concentrate alleviates epididymis and sperm damage in cadmium-intoxicated rats

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dc.citation.issue2
dc.citation.volume98
dc.contributor.authorLamas, Celina de A.
dc.contributor.authorCuquetto-Leite, Livia
dc.contributor.authorda Silva, Emanueli do Nascimento
dc.contributor.authorThomazini, Bruna F.
dc.contributor.authorCordeiro, Gabriel da S.
dc.contributor.authorPredes, Fabricia de S.
dc.contributor.authorGolluecke, Andrea P. B. [UNIFESP]
dc.contributor.authorDolder, Heidi
dc.coverageHoboken
dc.date.accessioned2020-07-17T14:02:23Z
dc.date.available2020-07-17T14:02:23Z
dc.date.issued2017
dc.description.abstractThe possibility of long-term grape juice concentrate (GJC) consumption conferring a protective effect against cadmium (Cd)-induced damage to the epididymis, completely preserving sperm profile, was evaluated here for the first time in the scientific literature. Male Wistar rats (n=6/per group) received an intraperitoneal Cd injection (1.2mg/Kg) at age 80days and GJC (2g/Kg) by gavage from 50days until 136days old. Groups receiving either Cd or GJC were added. An intraperitoneal injection of saline (0.9%) and water by gavage was administered in the absence of treatment with Cd or GJC. Animals were anaesthetized and exsanguinated at 136daysen
dc.description.abstractthe vas deferens, left testis and epididymis were removeden
dc.description.abstractand perfusion continued with fixative. The right epididymis was collected for morphological analysis. Cd had a devastating effect demonstrated by reduced sperm count in testes and epididymis, sperm production and normal sperm count, besides increased epididymis sperm transit time and completely disorganized morphology. These alterations were attributed to higher Cd levels in the testes and a lipid peroxidation (LP) process. Consumption of GJC plus Cd intoxication was effective, reducing metal accumulation and LP. Consequently, we could identify a preserved sperm profile, with improvement in testis and epididymis sperm count, normal sperm structure and sperm transit time. Moreover, GJC extends its protective effect to the epididymis, allowing complete re-establishment of its morphology, ensuring successful sperm maturation process. In conclusion, our study indicates long-term GJC as a promising therapy against reproductive chemical intoxication injury damage, preserving sperm prior to ejaculation.en
dc.description.affiliationUniv Estadual Campinas, Biol Inst, Dept Struct & Funct Biol, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Chem Inst, Dept Analyt Chem, Campinas, SP, Brazil
dc.description.affiliationState Univ Parana, Dept Biol Sci, Campus Paranagua, Paranagua, PR, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel (Capes)
dc.format.extent86-99
dc.identifierhttp://dx.doi.org/10.1111/iep.12227
dc.identifier.citationInternational Journal Of Experimental Pathology. Hoboken, v. 98, n. 2, p. 86-99, 2017.
dc.identifier.doi10.1111/iep.12227
dc.identifier.issn0959-9673
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54781
dc.identifier.wosWOS:000404372100004
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofInternational Journal Of Experimental Pathology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectheavy metalen
dc.subjectmale intoxicationen
dc.subjectmale reproductionen
dc.subjectmorphometryen
dc.subjectpolyphenolsen
dc.titleGrape juice concentrate alleviates epididymis and sperm damage in cadmium-intoxicated ratsen
dc.typeinfo:eu-repo/semantics/article
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