B-1 cells promote immunosurveillance against murine melanoma in host absence of CCR5: New perspective in autologous vaccination therapy

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dc.contributor.authorVivanco, Bruno C. [UNIFESP]
dc.contributor.authorViana, Jacqueline D. [UNIFESP]
dc.contributor.authorPerez, Elisabeth C.
dc.contributor.authorKonno, Fabiana T. C.
dc.contributor.authorGuereschi, Marcia G. [UNIFESP]
dc.contributor.authorXander, Patricia [UNIFESP]
dc.contributor.authorKeller, Alexandre C. [UNIFESP]
dc.contributor.authorLopes, Jose D. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Paulista
dc.date.accessioned2016-01-24T14:38:05Z
dc.date.available2016-01-24T14:38:05Z
dc.date.issued2014-11-01
dc.description.abstractAutologous vaccination with tumor-primed dendritic cells increases immune response against tumor, which seems to be improved in host absence of CCR5. Because B-1 lymphocytes modulate the activity of different immune cells, we decided to study their influence in the resistance against murine B16F10 melanoma in a CCR5 deprived environment. Adoptive transfer of peritoneal B-1 CCR5(+/+) lymphocytes to CCR5(-/-) animals inhibited the establishment of lung metastasis and melanoma cell growth, in comparison to saline-treated CCR5-/- mice. in loco cell analysis demonstrated that the adoptive transfer of B-1 CCR5(+/+) + lymphocytes to CCR5 deficient host was associated with a more intense influx of T CD8(+) to tumor site, indicating that the presence of CCR5(+/+) B-1 cells in the tumor environment induces the migration of T CD8 CCR5(-/-) cells to the implantation site. To corroborate this idea, CCR5(-/-) mice were injected with non B-1 peritoneal cells from wild type (WT) mice before B16F10 inoculation. in this regimen, CCR5(-/-) mice were not protected from tumor growth reinforcing the idea that, in host absence of CCR5, B-1 cells are essential to confer tumor resistance. This work indicates that, in the host absence of CCR5, naive B-1 cells may activate CD8T lymphocytes thereby promoting tumor resistance. Our results strongly suggest that autologous vaccination with B-1 lymphocytes in combination with CCR5 antagonists can be an alternative approach to tumor therapy. (C) 2014 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Paulista, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Pesquisa e Desenvolvimento, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDConselho Nacional de Pesquisa e Desenvolvimento, Brazil: 303858/2012-5
dc.description.sponsorshipIDConselho Nacional de Pesquisa e Desenvolvimento, Brazil: 301335/2009-5
dc.description.sponsorshipIDFAPESP: 11/50256-6
dc.format.extent845-849
dc.identifierhttp://dx.doi.org/10.1016/j.imbio.2014.07.013
dc.identifier.citationImmunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 219, n. 11, p. 845-849, 2014.
dc.identifier.doi10.1016/j.imbio.2014.07.013
dc.identifier.issn0171-2985
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38396
dc.identifier.wosWOS:000344831700005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofImmunobiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectB-1 lymphocytesen
dc.subjectB16F10 melanomaen
dc.subjectTumoren
dc.subjectCCR5en
dc.subjectAutologous vaccinationen
dc.titleB-1 cells promote immunosurveillance against murine melanoma in host absence of CCR5: New perspective in autologous vaccination therapyen
dc.typeinfo:eu-repo/semantics/article
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