Spectroscopic and structural analysis of somatic and N-domain angiotensin I-converting enzyme isoforms from mesangial cells from Wistar and spontaneously hypertensive rats

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dc.contributor.authorAndrade, Maria C. C. de [UNIFESP]
dc.contributor.authorAffonso, Regina [UNIFESP]
dc.contributor.authorFernandes, Fernanda B. [UNIFESP]
dc.contributor.authorFebba, Andreia C. [UNIFESP]
dc.contributor.authorSilva, Ismael D. C. G. da [UNIFESP]
dc.contributor.authorStella, Regina C. R. [UNIFESP]
dc.contributor.authorMarson, Odair [UNIFESP]
dc.contributor.authorJubilut, Guita N. [UNIFESP]
dc.contributor.authorHirata, Izaura Y. [UNIFESP]
dc.contributor.authorCarmona, Adriana K. [UNIFESP]
dc.contributor.authorCorradi, Hazel
dc.contributor.authorAcharya, K. Ravi
dc.contributor.authorSturrock, Edward D.
dc.contributor.authorCasarini, Dulce E. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionIPEN CNEN SP
dc.contributor.institutionUniv Bath
dc.contributor.institutionUniv Cape Town
dc.date.accessioned2016-01-24T14:05:16Z
dc.date.available2016-01-24T14:05:16Z
dc.date.issued2010-08-01
dc.description.abstractAngiotensin I-converting enzyme (ACE) plays a key role in the renin-angiotesin aldosterone cascade. We analysed the secondary structure and structural organization of a purified 65 kDa N-domain ACE (nACE) from Wistar rat mesangial cells, a 90 kDa nACE from spontaneously hypertensive rats and a 130 kDa somatic ACE. the C-terminal alignment of the 65 kDa nACE with rat ACE revealed that the former was truncated at Ser(482), and the sequence of the 90 kDa nACE ended at Pro(629). Protein's secondary structure consisted predominantly of a-helices. the 90 and 65 kDa isoforms were the most stable in guanidine and at low pH, respectively. Enzymatic activity decreased with loss in secondary structure, except in the case of guanidine HCl where the 90 kDa fragment loses its secondary structure faster than its enzymatic activity. We identified and characterized the activity and stability of these isoforms and these findings would be helpful on the understanding of the role of nACE isoforms in hypertension. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Disciplina Nefrol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Obstet & Ginecol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, Brazil
dc.description.affiliationIPEN CNEN SP, São Paulo, Brazil
dc.description.affiliationUniv Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
dc.description.affiliationUniv Cape Town, Div Med Biochem, ZA-7925 Observatory, South Africa
dc.description.affiliationUniv Cape Town, Inst Infect Dis & Mol Med, ZA-7925 Observatory, South Africa
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Disciplina Nefrol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Obstet & Ginecol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipLaboratorio Nacional de Luz Sincroton, Campinas, Brazil
dc.description.sponsorshipMedical Research Council, UK
dc.description.sponsorshipIDFAPESP: 03/02575-9
dc.description.sponsorshipIDFAPESP: 02/13290-2
dc.description.sponsorshipIDCNPq: 472055/2009-7
dc.description.sponsorshipIDMedical Research Council, UK: 81272
dc.format.extent238-243
dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2010.04.015
dc.identifier.citationInternational Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 47, n. 2, p. 238-243, 2010.
dc.identifier.doi10.1016/j.ijbiomac.2010.04.015
dc.identifier.issn0141-8130
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32778
dc.identifier.wosWOS:000279999200026
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAngiotensin I-converting enzymeen
dc.subjectN-domain ACE isoformsen
dc.subjectStructure analysisen
dc.subjectCircular dichroismen
dc.subjectHypertensionen
dc.subjectInhibitor designen
dc.titleSpectroscopic and structural analysis of somatic and N-domain angiotensin I-converting enzyme isoforms from mesangial cells from Wistar and spontaneously hypertensive ratsen
dc.typeinfo:eu-repo/semantics/article
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