In Vitro Evaluation of Chloroaluminum Phthalocyanine Nanoemulsion and Low-Level Laser Therapy on Human Skin Dermal Equivalents and Bone Marrow Mesenchymal Stem Cells

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dc.contributor.authorPrimo, F. L.
dc.contributor.authorReis, M. B. da Costa [UNIFESP]
dc.contributor.authorPorcionatto, Marimélia Aparecida [UNIFESP]
dc.contributor.authorTedesco, Antonio Claudio [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:05:13Z
dc.date.available2018-06-15T17:05:13Z
dc.date.issued2011-08-01
dc.description.abstractNanotechnology and tissue engineering are promising scientific fields in the development of advanced materials useful to human health. This article describes the preparation of a nanocarrier for the controlled release of a photosensitizer compound associated with low-level light therapy for skin wound healing treatment and applicable to other skin diseases. A biological model was used as an in vitro skin equivalent based on a three-dimensional culture of fibroblasts and mesenchymal stem cells and denominated by dermal equivalent (DE). Results show that it is possible to use the photomodulation process to control the wound healing in a scratching process and to induce the biomolecules release, both of which are related with the inflammatory wound healing process. In the studies, the MMP-2 and MMP-9 expression from zymography analyses were evaluated. All results showed a dependence on enzymatic activity relating to low-level laser applications which indicates a potential application in wound healing processes based on phototherapy and nanotechnology.en
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Lab Fotobiol & Fotomed, Ctr Nanotecnol & Engn Tecidual,Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2009/15363-9
dc.description.sponsorshipIDFAPESP: 2008/53719-4
dc.description.sponsorshipIDFAPESP: 2007/55319-0
dc.description.sponsorshipIDFAPESP: 2006/50562-1
dc.format.extent3376-3381
dc.identifierhttp://dx.doi.org/10.2174/092986711796504745
dc.identifier.citationCurrent Medicinal Chemistry. Sharjah: Bentham Science Publ Ltd, v. 18, n. 22, p. 3376-3381, 2011.
dc.identifier.doi10.2174/092986711796504745
dc.identifier.issn0929-8673
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43448
dc.identifier.wosWOS:000294405600012
dc.language.isoeng
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCurrent Medicinal Chemistry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDermal equivalentsen
dc.subjectmesenchymal stem cellsen
dc.subjectnanoemulsionsen
dc.subjectnanotechnologyen
dc.subjectphthalocyanineen
dc.subjecttissue engineeringen
dc.subjectphotodynamic processen
dc.subjecthuman fibroblasten
dc.subjectlow-level laser therapyen
dc.subjectmatrix metalloproteinasesen
dc.titleIn Vitro Evaluation of Chloroaluminum Phthalocyanine Nanoemulsion and Low-Level Laser Therapy on Human Skin Dermal Equivalents and Bone Marrow Mesenchymal Stem Cellsen
dc.typeinfo:eu-repo/semantics/article
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