Direct electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric support

Página do item simplificado
dc.contributor.authorNakaie, Clovis R.
dc.contributor.authorMalavolta, Luciana
dc.contributor.authorSchreier, Shirley
dc.contributor.authorTrovatti, Eliane
dc.contributor.authorMarchetto, Reinaldo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUNESP
dc.date.accessioned2016-01-24T12:41:15Z
dc.date.available2016-01-24T12:41:15Z
dc.date.issued2006-06-14
dc.description.abstractThis work demonstrates, for the first time. a time-resolved electron paramagnetic resonance (EPR) monitoring of a chemical reaction occurring in a polymeric structure. the progress of the coupling of a N-alpha-tert-butyloxycarbonyl-2.2.6.6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (Boc-TOAC) spin probe to a model peptide-resin was followed through EPR spectra. Progressive line broadening of EPR peaks was observed, indicative of an increased population of immobilized spin probe molecules attached to the solid support. the time for spectral stabilization of this process coincided with that determined in a previous Coupling study. thereby validating this in situ quantitative monitoring of the reaction. in addition, the influence of polymer swelling degree and solvent viscosity, as well as of the steric hindrance within beads. on the rate of coupling reaction was also addressed. A deeper evaluation of the latter effect was possible by determining unusual polymer parameters such as the average site-site distance and site-concentration within resin beads in each solvent system. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Chem, Dept Biochem, BR-05513970 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Biochem & Technol Chem, Inst Chem, BR-14800900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent4531-4536
dc.identifierhttp://dx.doi.org/10.1016/j.polymer.2006.05.007
dc.identifier.citationPolymer. Oxford: Elsevier B.V., v. 47, n. 13, p. 4531-4536, 2006.
dc.identifier.doi10.1016/j.polymer.2006.05.007
dc.identifier.issn0032-3861
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28976
dc.identifier.wosWOS:000238921300010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPolymer
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectpeptideen
dc.subjectpolymeren
dc.subjectelectron paramagnetic resonanceen
dc.titleDirect electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric supporten
dc.typeArtigo
Arquivos
Coleções
Em verificação - Geral