Análise da expressão de NRN1 e TNFAIP3 no sangue periférico de pacientes com câncer gástrico
Data
2022-04-29
Autores
Queiroz, Daniel Soares de 
Orientadores
Santos, Fernanda Wisnieski Caires dos
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O câncer gástrico (CG) é frequentemente diagnosticado em estádios avançados devido à ausência de sintomas ou à presença de sintomas não específicos nos estádios iniciais. A identificação de marcadores de diagnóstico e prognóstico confiáveis e minimamente invasivos é extremamente necessária para o aumento da sobrevida, assim como já ocorre em outros tipos tumorais. Dados prévios do nosso grupo de pesquisa revelaram NRN1 e TNFAIP3 como potenciais biomarcadores de diagnóstico/prognóstico e de resposta terapêutica em amostras de tecido gástrico neoplásico. Objetivos: avaliar se os níveis transcricionais dos genes NRN1 e TNFAIP3 podem constituir potenciais biomarcadores minimamente invasivos de diagnóstico e prognóstico, bem como de monitoramento dos pacientes. Métodos: os níveis transcricionais de NRN1 e TNFAIP3 a partir de amostras de sangue periférico de 42 participantes com CG e de 12 indivíduos sem câncer e negativos para H. pylori foram quantificados por RTqPCR. Um estudo de seguimento foi conduzido em 11 participantes com CG para monitorar os níveis de NRN1 e TNFAIP3 no período de até cinco anos após o tratamento cirúrgico. Os dados de expressão gênica foram associados às variáveis clínicas e patológicas. Resultados: os participantes com CG apresentaram maiores níveis transcricionais de NRN1 em relação aos indivíduos sem câncer (P<0,01). Além disso, a expressão de NRN1 foi capaz de discriminar esses indivíduos (AUC=0,72, P=0,02). Redução dos níveis transcricionais de NRN1 foi observada nos participantes com CG após a ressecção cirúrgica, alcançando os níveis detectados nos indivíduos sem câncer e sendo mais significativa um ano após o tratamento. Não foram detectadas alterações nos níveis de TNFAIP3 nos participantes com CG em relação aos controles, bem como após o tratamento cirúrgico. Conclusões: a expressão de NRN1 no sangue periférico tem o potencial para ser utilizado como um biomarcador de diagnóstico do CG e no monitoramento dos pacientes.
Gastric cancer (GC) is often diagnosed at advanced stages due to the absence of symptoms or the presence of nonspecific symptoms at early tumor stages. The identification of reliable and noninvasive diagnostic and prognostic markers is extremely necessary to increase survival, as already observed in other tumor types. Previous data from our research group revealed NRN1 and TNFAIP3 as potential diagnostic/prognostic and therapeutic response biomarkers in neoplastic gastric tissue samples. Objectives: to evaluate whether the transcriptional levels of the NRN1 and TNFAIP3 may constitute potential noninvasive biomarkers for GC diagnosis, prognosis, and patient monitoring. Methods: transcriptional levels of NRN1 and TNFAIP3 of peripheral blood samples from 42 subjects with GC and 12 subjects without cancer and negative for H. pylori were quantified by RTqPCR. A follow-up study was conducted in 11 participants with GC to monitor NRN1 and TNFAIP3 levels for up to five years after surgical treatment. Gene expression data were associated with clinical and pathological variables. Results: participants with GC had higher transcriptional levels of NRN1 compared with individuals without cancer (P<0.01). Furthermore, the expression of NRN1 was able to discriminate against these individuals (AUC=0.72, P=0.02). Reduced NRN1 transcriptional levels were observed in participants with GC, reaching those previously observed in individuals without cancer, and being more significant one year after the treatment. No changes were detected in TNFAIP3 levels comparing participants with GC and individuals without cancer, nor in GC participants after surgical treatment. Conclusions: NRN1 expression has the potential to be used as a noninvasive biomarker for GC diagnosis and patient monitoring.
Gastric cancer (GC) is often diagnosed at advanced stages due to the absence of symptoms or the presence of nonspecific symptoms at early tumor stages. The identification of reliable and noninvasive diagnostic and prognostic markers is extremely necessary to increase survival, as already observed in other tumor types. Previous data from our research group revealed NRN1 and TNFAIP3 as potential diagnostic/prognostic and therapeutic response biomarkers in neoplastic gastric tissue samples. Objectives: to evaluate whether the transcriptional levels of the NRN1 and TNFAIP3 may constitute potential noninvasive biomarkers for GC diagnosis, prognosis, and patient monitoring. Methods: transcriptional levels of NRN1 and TNFAIP3 of peripheral blood samples from 42 subjects with GC and 12 subjects without cancer and negative for H. pylori were quantified by RTqPCR. A follow-up study was conducted in 11 participants with GC to monitor NRN1 and TNFAIP3 levels for up to five years after surgical treatment. Gene expression data were associated with clinical and pathological variables. Results: participants with GC had higher transcriptional levels of NRN1 compared with individuals without cancer (P<0.01). Furthermore, the expression of NRN1 was able to discriminate against these individuals (AUC=0.72, P=0.02). Reduced NRN1 transcriptional levels were observed in participants with GC, reaching those previously observed in individuals without cancer, and being more significant one year after the treatment. No changes were detected in TNFAIP3 levels comparing participants with GC and individuals without cancer, nor in GC participants after surgical treatment. Conclusions: NRN1 expression has the potential to be used as a noninvasive biomarker for GC diagnosis and patient monitoring.
Descrição
Citação
QUEIROZ, Daniel Soares de. Análise da expressão de NRN1e TNFAIP3 no sangue periférico de pacientes com câncer gástrico. 2022. 123 f. Dissertação (Mestrado em Gastroenterologia) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP). São Paulos, 2022.