Exhaled nitric oxide for monitoring childhood asthma inflammation compared to sputum analysis, serum interleukins and pulmonary function

dc.contributor.authorParo-Heitor, Maria Luisa Z.
dc.contributor.authorBussamra, Maria Helena C. F.
dc.contributor.authorSaraiva-Romanholo, Beatriz M.
dc.contributor.authorMartins, Milton A.
dc.contributor.authorOkay, Thelma Suely
dc.contributor.authorRodrigues, Joaquim Carlos
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:49:31Z
dc.date.available2016-01-24T13:49:31Z
dc.date.issued2008-02-01
dc.description.abstractThe level of fractional exhaled nitric oxide (FENO) is significantly elevated in uncontrolled asthma and decreases after anti-inflammatory therapy the aim of this prospective study was to analyze the behavior of FENO in the follow-up and management of the inflammation in asthmatic pediatric patients treated with inhaled corticosteroids (ICS), compared to sputum cellularity, serum interleukins (IL), and pulmonary function. Twenty-six clinically stable asthmatic children aged from 6 to 18 years, previously treated or not with ICS were included. Following an international consensus (GINA), the patients were submitted to standard treatment with inhaled fluticasone for 3 months according to the severity of the disease. During this period, each patient underwent three assessments at intervals of approximately 6 weeks: Each evaluation consisted of the measurement of FENO, determination of serum interleukins IL-5, IL-10, IL-13, and interferon gamma (INF-gamma), spirometry and cytological analysis of spontaneous or induced sputum. A significant reduction in mean FENO and IL-5, without concomitant changes in FEV1, was observed along the study. There was no significant correlation between FeNO and FEV1 in the three assessments. A significant correlation between FeNO and IL-5 levels was only observed in the third assessment (r = 0.499, P=0.025). in most patients, serum IL-10, IL-13, and INF-gamma concentrations were undetectable throughout the study Sputum samples were obtained spontaneously in 11 occasions and in 56 by induction with 3% hypertonic saline solution (success rate: 50.8%), with 39 (69.9%) of them adequate for analysis. Only two of the 26 patients produced adequate samples in the three consecutive evaluations, which impaired the determination of a potential association between sputum cellularity and FeNO levels throughout the study. in conclusion, among the parameters of this study, it was difficult to perform and to interpret the serial analysis of spontaneous or induced sputum. Serum interleukins, which remained at very low or undetectable levels in most patients, were not found to be useful for therapeutic monitoring, except for IL-5 that seems to present some correlation with levels of FeNO exhaled. Monitoring of the mean FEV1 indicated no significant variations during the treatment, demonstrating that functional stability or the absence of obstruction may not reflect the adequate management of asthma. Serial measurement of FeNO seemed to best reflect the progressive anti-inflammatory action of ICS in asthma.en
dc.description.affiliationUniv São Paulo, Sch Med, Hosp Clin,Pediat Pulmonol Unit, Inst Crianca,Unidade Pneumol,Dept Pediat,, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pathol & Med, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pediat, Lab Med Invest LIM 36, BR-05403900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent134-141
dc.identifierhttp://dx.doi.org/10.1002/ppul.20747
dc.identifier.citationPediatric Pulmonology. Hoboken: Wiley-liss, v. 43, n. 2, p. 134-141, 2008.
dc.identifier.doi10.1002/ppul.20747
dc.identifier.issn8755-6863
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30404
dc.identifier.wosWOS:000253401900004
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPediatric Pulmonology
dc.rightsAcesso restrito
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectairway inflammationen
dc.subjectchildhood asthmaen
dc.subjectfractional exhaled nitric oxideen
dc.subjectsputum analysisen
dc.subjectinterleukinsen
dc.subjectpulmonary functionen
dc.subjectinhaled corticosteroidsen
dc.titleExhaled nitric oxide for monitoring childhood asthma inflammation compared to sputum analysis, serum interleukins and pulmonary functionen
dc.typeArtigo
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