Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats

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dc.citation.volume8
dc.contributor.authorBanin, Renata Mancini [UNIFESP]
dc.contributor.authorAndrade, Iracema Senna de [UNIFESP]
dc.contributor.authorCerutti, Suzete Maria [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorTelles, Monica Marques [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-08-04T13:40:13Z
dc.date.available2020-08-04T13:40:13Z
dc.date.issued2017
dc.description.abstractMenopause is associated with increased risk to develop obesity but the mechanisms involved are not fully understood. We have shown that Ginkgo biloba extract (GbE) improved diet-induced obesity. Since GbE might be effective in the treatment of obesity related to menopause, avoiding the side effects of hormone replacement therapy, we investigated the effect of GbE on hypothalamic systems controlling energy homeostasis. Wistar rats were either ovariectomized (OVX) or Sham-operated. After 2 months, either 500 mg.kg(-1) of GbE or vehicle were administered daily by gavage for 14 days. A subset of animals received an intracerebroventricular (i.c.v.) injection of serotonin (300 mu g) or vehicle and food intake was measured after 12 and 24 h. Another subset was submitted to in vivo microdialysis and 5-HT levels of the medial hypothalamus were measured by high performance liquid chromatography, before and up to 2 h after the administration of 500 mg.kg(-1) of GbE. Additional animals were used for quantification of 5-HT1A, 5-HT1B, 5-HT2C, 5-HTT, and pro-opiomelanocortin hypothalamic protein levels by Western blotting. OVX increased food intake and body weight and adiposity while GbE attenuated these alterations. i.c.v. serotonin significantly reduced food intake in Sham, Sham + GbE, and OVX + GbE groups while it failed to do so in the OVX group. In the OVX rats, GbE stimulated 5-HT microdialysate levels while it reduced hypothalamic 5-HTT protein levels. The results indicate that GbE improved the ovariectomy-induced resistance to serotonin hypophagia, at least in part through stimulation of the hypothalamic serotonergic activity. Since body weight gain is one of the most important consequences of menopause, the stimulation of the serotonergic transmission by GbE may represent a potential alternative therapy for menopause-related obesity.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Biol, Setor Morfofisiol & Patol, Diadema, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencias Biol, Setor Morfofisiol & Patol, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipIDCNPq: 453924/2014-0
dc.description.sponsorshipIDFAPESP: 2012/03172-4
dc.description.sponsorshipIDFAPESP: 2014/18435-6
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.3389/fphar.2017.00605
dc.identifier.citationFrontiers In Pharmacology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fphar.2017.00605
dc.identifier.fileWOS000409382600001.pdf
dc.identifier.issn1663-9812
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57374
dc.identifier.wosWOS:000409382600001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Pharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOvariectomyen
dc.subjectObesityen
dc.subjectSerotoninen
dc.subjectHypothalamusen
dc.subjectFood intakeen
dc.subjectin vivo microdialysisen
dc.subjectGinkgo biloba extracten
dc.titleGinkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Ratsen
dc.typeinfo:eu-repo/semantics/article
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