Haplotype distribution of five nuclear genes based on network genealogies and Bayesian inference indicates that Trypanosoma cruzi hybrid strains are polyphyletic

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dc.contributor.authorTomazi, Laize [UNIFESP]
dc.contributor.authorKawashita, Silvia Yukie [UNIFESP]
dc.contributor.authorPereira, P. M. [UNIFESP]
dc.contributor.authorZingales, Bianca
dc.contributor.authorBriones, Marcelo Ribeiro da Silva [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-06-15T17:30:21Z
dc.date.available2018-06-15T17:30:21Z
dc.date.issued2009-01-01
dc.description.abstractChagas disease is still a major public health problem in Latin America. Its causative agent, Trypanosoma cruzi, can be typed into three major groups, T. cruzi I, T. cruzi II and hybrids. These groups each have specific genetic characteristics and epidemiological distributions. Several highly virulent strains are found in the hybrid group; their origin is still a matter of debate. The null hypothesis is that the hybrids are of polyphyletic origin, evolving independently from various hybridization events. The alternative hypothesis is that all extant hybrid strains originated from a single hybridization event. We sequenced both alleles of genes encoding EF-1 alpha, actin and SSU rDNA of 26 T. cruzi strains and DHFR-TS and TR of 12 strains. This information was used for network genealogy analysis and Bayesian phylogenies. We found T. cruzi I and T. cruzi II to be monophyletic and that all hybrids had different combinations of T. cruzi I and T. cruzi II haplotypes plus hybrid-specific haplotypes. Bootstrap values (networks) and posterior probabilities (Bayesian phylogenies) of clades supporting the monophyly of hybrids were far below the 95% confidence interval, indicating that the hybrid group is polyphyletic. We hypothesize that T. cruzi I and T. cruzi II are two different species and that the hybrids are extant representatives of independent events of genome hybridization, which sporadically have sufficient fitness to impact on the epidemiology of Chagas disease.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipInternational Research Scholars Program of the Howard Hughes Medical Institute (HHMI).
dc.format.extent458-476
dc.identifierhttp://dx.doi.org/10.4238/vol8-2gmr591
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 8, n. 2, p. 458-476, 2009.
dc.identifier.doi10.4238/vol8-2gmr591
dc.identifier.fileWOS000267938300011.pdf
dc.identifier.issn1676-5680
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43724
dc.identifier.wosWOS:000267938300011
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzien
dc.subjectChagas diseaseen
dc.subjectMolecular evolutionen
dc.subjectPhylogenyen
dc.subjectSmall subunit rDNAen
dc.titleHaplotype distribution of five nuclear genes based on network genealogies and Bayesian inference indicates that Trypanosoma cruzi hybrid strains are polyphyleticen
dc.typeinfo:eu-repo/semantics/article
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