Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium

dc.contributor.authorPereira, Marcos Fragoso de Noronha [UNIFESP]
dc.contributor.authorFernandes, Sheilla Alessandra Ferreira [UNIFESP]
dc.contributor.authorNascimento, Aline Rosa [UNIFESP]
dc.contributor.authorSiu, Erica Rosanna [UNIFESP]
dc.contributor.authorHess, Rex A.
dc.contributor.authorOliveira, Cleida Aparecida de
dc.contributor.authorPorto, Catarina Segreti [UNIFESP]
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Illinois
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.description.abstractThe role of oestrogens in epididymal function is still unclear. Knockout of the oestrogen receptor ESR1 (Esr1-/-) or treatment with the anti-oestrogen Fulvestrant affect epididymal milieu and sperm motility. We investigated the effect of in vivo treatment of rats with Fulvestrant on: (i) expression of genes that may be important for the architecture and function of the epididymal epithelium: prominins 1 and 2, metalloproteinase 7, claudin 7, beta-catenin and cadherin 13, and (ii) levels of oestradiol and testosterone, and expression of oestrogen and androgen receptors, in the initial segment (IS), caput, corpus and cauda epididymis. Fulvestrant (i) reduced gene expression of prominin 1 (variant 1) in the caput, reduced prominin 1 protein content in the caput epididymis and in the efferent ductules, and increased the localization of prominin 1 in microvilli of the caput and corpus; (ii) reduced gene expression of prominin 2 in the corpus and cauda epididymis; (iii) increased the metalloproteinase 7 content in the apical region of principal cells from IS/caput; (iv) reduced in the corpus epididymis, but increased in the efferent ductules, the cadherin 13 mRNA level; (v) reduced testosterone but increased oestradiol levels in the corpus and cauda; (vi) increased the androgen receptor protein content in all regions of the epididymis, and the oestrogen receptor GPER in the corpus and cauda epididymis. in conclusion, treatment with Fulvestrant induced regional-specific changes in hormonal and steroid receptor content, and affected expression of proteins important for epithelial organization and absorption/secretion. the mechanisms of oestrogen action may differ among epididymal regions, which may contribute to determine region-specific sperm functions.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Illinois, Dept Comparat Biosci, Urbana, IL 61801 USA
dc.description.affiliationUniv Fed Minas Gerais, Dept Morphol, Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipIDFAPESP: 2008/56564-1pt
dc.identifier.citationAndrology. Hoboken: Wiley-Blackwell, v. 2, n. 4, p. 559-571, 2014.
dc.rightsAcesso aberto
dc.subjectGene expressionen
dc.subjectAnimal modelsen
dc.titleEffects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epitheliumen