Construction of a recombinant Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV-2D) harbouring the beta-galactosidase gene

dc.contributor.authorRibeiro, B. M.
dc.contributor.authorGatti, CDC
dc.contributor.authorCosta, M. H.
dc.contributor.authorMoscardi, F.
dc.contributor.authorMaruniak, J. E.
dc.contributor.authorPossee, R. D.
dc.contributor.authorZanotto, PMA
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionNERC
dc.contributor.institutionUniv Florida
dc.date.accessioned2016-01-24T12:31:15Z
dc.date.available2016-01-24T12:31:15Z
dc.date.issued2001-01-01
dc.description.abstractWe have constructed a transfer vector (pAgGal) containing the beta -galactosidase gene under control of the Escherichia coli gpt and AgMNPV polyhedrin (polh) promoters. the transfer vector was cotransfected with wild type Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) DNA into A. gemmatalis (UFL-AG-286) cells and a recombinant baculovirus (vAgGalA2) was isolated. the beta -galactosidase gene insertion was checked by polymerase chain reaction (PCR) using DNA from AgMNPV and vAgGalA2 and primers specific for regions upstream and downstream of the polh gene. Insect cells (UFL-AG-286) were infected with the recombinant vAgGalA2 and wild type AgMNPV viruses and the production of the heterologous protein analyzed by SDS-PAGE and Pulse-Chase. beta -galactosidase was expressed at high levels late on infection as expected for a gene under the control of the polh promoter. the highly expressed beta -galactosidase protein was also shown to be biologically active by a beta -galactosidase assay.en
dc.description.affiliationUniv São Paulo, ICB, LEMB, UNIFESP,Dept Microbiol Imunol & Parasitol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Brasilia, Dept Biol Celular, Lab Cirol & Microscopia Eletron, Brasilia, DF, Brazil
dc.description.affiliationCNPSo, EMBRAPA, Londrina, PR, Brazil
dc.description.affiliationNERC, Inst Virol & Environm Microbiol, Oxford OX1 3SR, England
dc.description.affiliationUniv Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA
dc.description.affiliationUnifespUniv São Paulo, ICB, LEMB, UNIFESP,Dept Microbiol Imunol & Parasitol, BR-05508900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1355-1367
dc.identifierhttp://dx.doi.org/10.1007/s007050170096
dc.identifier.citationArchives of Virology. Vienna: Springer-verlag Wien, v. 146, n. 7, p. 1355-1367, 2001.
dc.identifier.doi10.1007/s007050170096
dc.identifier.issn0304-8608
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26444
dc.identifier.wosWOS:000170368200009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofArchives of Virology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.titleConstruction of a recombinant Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV-2D) harbouring the beta-galactosidase geneen
dc.typeArtigo
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