Exploring the genomic diversity of black yeasts and relatives (Chaetothyriales, Ascomycota)

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dc.citation.issue86
dc.contributor.authorTeixeira, M. M.
dc.contributor.authorMoreno, L. F.
dc.contributor.authorStielow, B. J.
dc.contributor.authorMuszewska, A.
dc.contributor.authorHainaut, M.
dc.contributor.authorGonzaga, L.
dc.contributor.authorAbouelleil, A.
dc.contributor.authorPatane, J. S. L.
dc.contributor.authorPriest, M.
dc.contributor.authorSouza, R.
dc.contributor.authorYoung, S.
dc.contributor.authorFerreira, K. S. [UNIFESP]
dc.contributor.authorZeng, Q.
dc.contributor.authorda Cunha, M. M. L.
dc.contributor.authorGladki, A.
dc.contributor.authorBarker, B.
dc.contributor.authorVicente, V. A.
dc.contributor.authorde Souza, E. M.
dc.contributor.authorAlmeida, S.
dc.contributor.authorHenrissat, B.
dc.contributor.authorVasconcelos, A. T. R.
dc.contributor.authorDeng, S.
dc.contributor.authorVoglmayr, H.
dc.contributor.authorMoussa, T. A. A.
dc.contributor.authorGorbushina, A.
dc.contributor.authorFelipe, M. S. S.
dc.contributor.authorCuomo, C. A.
dc.contributor.authorde Hoog, G. Sybren
dc.coverageUtrecht
dc.date.accessioned2020-07-17T14:02:41Z
dc.date.available2020-07-17T14:02:41Z
dc.date.issued2017
dc.description.abstractThe order Chaetothyriales (Pezizomycotina, Ascomycetes) harbours obligatorily melanised fungi and includes numerous etiologic agents of chromoblastomycosis, phaeohyphomycosis and other diseases of vertebrate hosts. Diseases range from mild cutaneous to fatal cerebral or disseminated infections and affect humans and cold-blooded animals globally. In addition, Chaetothyriales comprise species with aquatic, rock-inhabiting, ant-associated, and mycoparasitic life-styles, as well as species that tolerate toxic compounds, suggesting a high degree of versatile extremotolerance. To understand their biology and divergent niche occupation, we sequenced and annotated a set of 23 genomes of main the human opportunists within the Chaetothyriales as well as related environmental species. Our analyses included fungi with diverse life-styles, namely opportunistic pathogens and closely related saprobes, to identify genomic adaptations related to pathogenesis. Furthermore, ecological preferences of Chaetothyriales were analysed, in conjuncture with the order-level phylogeny based on conserved ribosomal genes. General characteristics, phylogenomic relationships, transposable elements, sex-related genes, protein family evolution, genes related to protein degradation (MEROPS), carbohydrate-active enzymes (CAZymes), melanin synthesis and secondary metabolism were investigated and compared between species. Genome assemblies varied from 25.81 Mb (Capronia coronata) to 43.03 Mb (Cladophialophora immunda). The bantiana-clade contained the highest number of predicted genes (12 817 on average) as well as larger genomes. We found a low content of mobile elements, with DNA transposons from Tc1/Mariner superfamily being the most abundant across analysed species. Additionally, we identified a reduction of carbohydrate degrading enzymes, specifically many of the Glycosyl Hydrolase (GH) class, while most of the Pectin Lyase (PL) genes were lost in etiological agents of chromoblastomycosis and phaeohyphomycosis. An expansion was found in protein degrading peptidase enzyme families S12 (serine-type D-Ala-D-Ala carboxypeptidases) and M38 (isoaspartyl dipeptidases). Based on genomic information, a wide range of abilities of melanin biosynthesis was revealeden
dc.description.abstractgenes related to metabolically distinct DHN, DOPA and pyomelanin pathways were identified. The MAT (MAting Type) locus and other sex-related genes were recognized in all 23 black fungi. Members of the asexual genera Fonsecaea and Cladophialophora appear to be heterothallic with a single copy of either MAT-1-1 or MAT-1-2 in each individual. All Capronia species are homothallic as both MAT1-1 and MAT1-2 genes were found in each single genome. The genomic synteny of the MAT-locus flanking genes (SLA2-APN2-COX13) is not conserved in black fungi as is commonly observed in Eurotiomycetes, indicating a unique genomic context for MAT in those species. The heterokaryon (het) genes expansion associated with the low selective pressure at the MAT-locus suggests that a parasexual cycle may play an important role in generating diversity among those fungi.en
dc.description.affiliationTranslat Genom Res Inst TGen, Div Pathogen Gen, Flagstaff, AZ USA
dc.description.affiliationUniv Brasilia, Dept Cell Biol, Brasilia, DF, Brazil
dc.description.affiliationWesterdijk Fungal Biodivers Inst, Utrecht, Netherlands
dc.description.affiliationPolish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland
dc.description.affiliationUniv Aix Marseille, CNRS, Marseille, France
dc.description.affiliationNatl Lab Sci Comp LNCC, Petropolis, Brazil
dc.description.affiliationBroad Inst MIT & Harvard, Cambridge, MA 02142 USA
dc.description.affiliationUniv Sao Paulo, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Diadema, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Nucleo Multidisciplinar Pesquisa Biol UFRJ Xerem, Rio De Janeiro, Brazil
dc.description.affiliationUniv Fed Parana, Dept Basic Pathol, Curitiba, PR, Brazil
dc.description.affiliationUniv Fed Parana, Dept Biochem & Mol Biol, Curitiba, PR, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Clin & Toxicol Anal, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
dc.description.affiliationSecond Mil Med Univ, Shanghai Inst Med Mycol, Changzheng Hosp, Shanghai, Peoples R China
dc.description.affiliationniv Vienna, Dept Systemat & Evolutionary Bot, Vienna, Austria
dc.description.affiliationKing Abdulaziz Univ, Fac Sci, Biol Sci Dept, Jeddah, Saudi Arabia
dc.description.affiliationCairo Univ, Fac Sci, Bot & Microbiol Dept, Giza, Egypt
dc.description.affiliationBAM Fed Inst Mat Res & Testing, Berlin, Germany
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biol Sci, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Institute of Science and Technology of Nitrogen Fixation/CNPq/MCT
dc.description.sponsorshipFundacao Araucaria
dc.description.sponsorshipNational Human Genome Research Institute
dc.description.sponsorshipCNPq/FAP-DF
dc.description.sponsorshipCAPES-Brazil
dc.description.sponsorshipKing Abdulaziz University, Jeddah
dc.description.sponsorshipDSR
dc.description.sponsorshipNational Science Centre (Poland)
dc.description.sponsorshipLNCC
dc.description.sponsorshipIDNational Institute of Science and Technology of Nitrogen Fixation/CNPq/MCT: 573828/2008-3
dc.description.sponsorshipIDNational Human Genome Research Institute: U54HG003067
dc.description.sponsorshipIDCNPq/FAP-DF: 193000569/2009
dc.description.sponsorshipIDCAPES: 1331-13-2
dc.description.sponsorshipIDKing Abdulaziz University, Jeddah: 30-130-36-RG
dc.description.sponsorshipIDNational Science Centre (Poland): 2012/07/D/NZ2/04286
dc.format.extent1-28
dc.identifierhttp://dx.doi.org/10.1016/j.simyco.2017.01.001
dc.identifier.citationStudies In Mycology. Utrecht, v. , n. 86, p. 1-28, 2017.
dc.identifier.doi10.1016/j.simyco.2017.01.001
dc.identifier.fileWOS000411880700001.pdf
dc.identifier.issn0166-0616
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54944
dc.identifier.wosWOS:000411880700001
dc.language.isoeng
dc.publisherCentraalbureau Schimmelculture
dc.relation.ispartofStudies In Mycology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBlack yeasten
dc.subjectComparative genomicsen
dc.subjectChaetothyrialesen
dc.subjectEcologyen
dc.subjectEvolutionen
dc.subjectHerpotrichiellaceaeen
dc.subjectPhylogenyen
dc.titleExploring the genomic diversity of black yeasts and relatives (Chaetothyriales, Ascomycota)en
dc.typeinfo:eu-repo/semantics/article
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