Molecular analysis of clinical isolates of Providencia alcalifaciens

dc.contributor.authorSobreira, M.
dc.contributor.authorLeal, N. C.
dc.contributor.authorMagalhaes, M.
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorAlmeida, AMP
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractIn an attempt to elucidate the virulence factors and the pathogenic mechanisms of Providencia alcalifaciens, 36 isolates identified in 1994-1995 in Recife city, Brazil were analysed by PCR to investigate the presence of DNA sequences homologous to virulence genes described in other invasive enterobacteria, as well as their ability to invade HeLa cells, their plasmid profiles and antibiotic resistance patterns. The genetic diversity of the isolates was also analysed by RAPD-PCR. pile homologous sequences of virulence genes were observed with any of the P. alcalifaciens isolates studied. Ten isolates had no plasmid and 26 harboured one-to-Eve plasmids of 147-<6.9 kb. Invasion of HeLa cells was observed in only 10 isolates. No correlation between the plasmid content of the strains, their invasion of HeLa cells or their resistance to antimicrobial drugs could be established. The isolates could be distributed into 10 genotypic groups by RAPD-PCR. Considering the genotypic profile and ability to invade HeLa cells, 7 of the 10 invasive isolates belonged to the same genotypic group. The presence of invasive isolates in the same or a related genotypic group suggests the existence of a clonal lineage responsible for the invasiveness.en
dc.description.affiliationUniv Fed Pernambuco, Lab Immunopathol Keizo Asami, Recife, PE, Brazil
dc.description.affiliationFiocruz MS, Ctr Pesquisas Aggeu Magalhaes, Dept Microbiol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Disciplina Microbiol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Disciplina Microbiol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationJournal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 50, n. 1, p. 29-34, 2001.
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofJournal Of Medical Microbiology
dc.rightsAcesso aberto
dc.titleMolecular analysis of clinical isolates of Providencia alcalifaciensen