Effects of Pentoxifylline on Inflammation and Lung Dysfunction in Ventilated Septic Animals

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dc.contributor.authorOliveira-Junior, Itamar Souza [UNIFESP]
dc.contributor.authorSouza Oliveira, Wagner Rogerio [UNIFESP]
dc.contributor.authorCavassani, Samia Santos [UNIFESP]
dc.contributor.authorColo Brunialti, Milena Karina
dc.contributor.authorSalomao, Reinaldo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:59:33Z
dc.date.available2016-01-24T13:59:33Z
dc.date.issued2010-04-01
dc.description.abstractAcute respiratory distress syndrome secondary to sepsis is associated with high morbidity and mortality. the purpose of this study was to characterize the effects of ventilatory strategy and the modulating activity of pentoxifylline in a sepsis-induced lung dysfunction model. Male Wistar rats were randomly divided into six groups, undergoing two different ventilatory strategies. Rats received live Escherichia coli or saline intraperitoneally. After 6 hours, the septic animals were treated with either pentoxifylline (25 mg/kg for 20 minutes) or normal saline infusion and ventilated with low tidal volume (6 mL/kg; septic animals with E. coli intraperitoneal [IP] infusion, PTX-treated and ventilated with low tidal volume and septic animals with E. coli IP infusion and ventilated with low tidal volume, respectively) or high tidal volume (12 mL/kg; septic animals with E. coli IP infusion, PTX-treated and ventilated with high tidal volume and septic animals with E. coli IP infusion and ventilated with high tidal volume, respectively) for 3 hours. the control animals received normal saline infusion and, after 6 hours, were ventilated with low or high tidal volume (control animals with saline infusion and ventilated with low tidal volume and control animals with saline infusion and ventilated with high tidal volume, respectively). Lung dysfunctions were assessed by wet-to-dry lung ratios, total cell count, total protein, malondialdehyde, and tumor necrosis factor-alpha concentrations in bronchoalveolar lavage (BAL). Septic animals with E. coli IP infusion and ventilated with high tidal volume presented increased wet-to-dry lung ratios, total cell count, total protein, and malondialdehyde in BAL compared with the septic animals ventilated with low tidal volume. Septic animals treated with pentoxifylline presented higher arterial oxygenation and lower cellular influx, protein leakage, malondialdehyde concentration, and tumor necrosis factor-alpha levels in BAL compared with septic animals undergoing the same ventilatory support strategies (septic animals with E. coli IP infusion and ventilated with low tidal volume and septic animals with E. coli IP infusion and ventilated with high tidal volume). Ventilatory strategy modulated the inflammatory response and pulmonary alterations in a sepsis-induced acute lung injury model, and these effects are improved by pentoxifylline.en
dc.description.affiliationUniversidade Federal de São Paulo, Immunol Lab, Escola Paulista Med, Div Infect Dis, BR-04039032 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Div Histol & Struct Biol, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Immunol Lab, Escola Paulista Med, Div Infect Dis, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Div Histol & Struct Biol, BR-04039032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado São Paulo
dc.description.sponsorshipIDFundacao de Amparo a Pesquisa do Estado São Paulo: 03/14098-0
dc.format.extent822-826
dc.identifierhttp://dx.doi.org/10.1097/TA.0b013e3181a5f4b5
dc.identifier.citationJournal of Trauma-injury Infection and Critical Care. Philadelphia: Lippincott Williams & Wilkins, v. 68, n. 4, p. 822-826, 2010.
dc.identifier.doi10.1097/TA.0b013e3181a5f4b5
dc.identifier.issn0022-5282
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32446
dc.identifier.wosWOS:000276663100014
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofJournal of Trauma-injury Infection and Critical Care
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectLung injuryen
dc.subjectSepsisen
dc.subjectLow tidal volumeen
dc.subjectHigh tidal volumeen
dc.subjectPentoxifyllineen
dc.subjectTNF-alphaen
dc.subjectInflammatory responseen
dc.titleEffects of Pentoxifylline on Inflammation and Lung Dysfunction in Ventilated Septic Animalsen
dc.typeinfo:eu-repo/semantics/article
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