Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats

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dc.contributor.authorLeite, Aline de Lima
dc.contributor.authorSantiago, Joel Ferreira
dc.contributor.authorLevy, Flavia Mauad
dc.contributor.authorMaria, Andrea Gutierrez
dc.contributor.authorFernandes, Mileni da Silva
dc.contributor.authorSalvadori, Daisy Maria Favero
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorRabelo Buzalaf, Marflia Afonso
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:48:43Z
dc.date.available2016-01-24T13:48:43Z
dc.date.issued2007-05-01
dc.description.abstractFluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. in the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. the level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure.en
dc.description.affiliationUniv São Paulo, Dept Biol Sci, Bauru Dent Sch, BR-17012901 Bauru, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Pathol, Botucatu Med Sch, São Paulo, Brazil
dc.description.affiliationUNIFESP, Dept Hlth Sci, Santos, SP, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Hlth Sci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent435-440
dc.identifierhttps://dx.doi.org/10.1177/0960327107076288
dc.identifier.citationHuman & Experimental Toxicology. London: Sage Publications Ltd, v. 26, n. 5, p. 435-440, 2007.
dc.identifier.doi10.1177/0960327107076288
dc.identifier.issn0960-3271
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/29743
dc.identifier.wosWOS:000248156000006
dc.language.isoeng
dc.publisherSage Publications Ltd
dc.relation.ispartofHuman & Experimental Toxicology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.uk.sagepub.com/aboutus/openaccess.htm
dc.subjectAcute exposureen
dc.subjectComet assayen
dc.subjectDNA damageen
dc.subjectFluorideen
dc.subjectRatsen
dc.titleAbsence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in ratsen
dc.typeinfo:eu-repo/semantics/article
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