A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer

Página do item simplificado
dc.contributor.authorHu, Yuntao
dc.contributor.authorLiu, Laura
dc.contributor.authorLu, Bo
dc.contributor.authorZhu, Danhong
dc.contributor.authorRibeiro, Ramiro
dc.contributor.authorDiniz, Bruno [UNIFESP]
dc.contributor.authorThomas, Padmaja B.
dc.contributor.authorAhuja, Ashish K.
dc.contributor.authorHinton, David R.
dc.contributor.authorTai, Yu-Chong
dc.contributor.authorHikita, Sherry T.
dc.contributor.authorJohnson, Lincoln V.
dc.contributor.authorClegg, Dennis O.
dc.contributor.authorThomas, Biju B.
dc.contributor.authorHumayun, Mark S.
dc.contributor.institutionUniv So Calif
dc.contributor.institutionCALTECH
dc.contributor.institutionUniv Calif Santa Barbara
dc.contributor.institutionPeking Univ
dc.contributor.institutionChang Gung Mem Hosp
dc.contributor.institutionHosp Evangel Curitiba
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:38Z
dc.date.available2016-01-24T14:17:38Z
dc.date.issued2012-01-01
dc.description.abstractObjective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. Methods: A platform device was used for the implantation of 4-mu m-thick parylene substrates containing a monolayer of human embryonic stem cell-derived RPE (hESC-RPE). Normal Copenhagen rats (n = 6) and RCS rats (n = 5) were used for the study. Spectral-domain optical coherence tomography (SD-OCT) scanning and histological examinations were performed to confirm placement location of the implant. hESC- RPE cells attached to the substrate before and after implantation were evaluated using standard cell counting techniques. Results: SD-OCT scanning and histological examination revealed that the substrates were precisely placed in the rat's subretinal space. the hESC-RPE cell monolayer that covered the surface of the substrate was found to be intact after implantation. Cell counting data showed that less than 2% of cells were lost from the substrate due to the implantation procedure (preimplantation count 2,792 +/- 74.09 cells versus postimplantation count 2,741 +/- 62.08 cells). Detailed microscopic examination suggested that the cell loss occurred mostly along the edges of the implant. Conclusion: With the help of this platform device, it is possible to implant ultrathin substrates containing an RPE monolayer into the rat's subretinal space. This technique can be a useful approach for stem cell-based tissue bioengineering techniques in retinal transplantation research. Copyright (c) 2012 S. Karger AG, Baselen
dc.description.affiliationUniv So Calif, Doheny Eye Inst, Keck Sch Med, Los Angeles, CA 90033 USA
dc.description.affiliationUniv So Calif, Dept Ophthalmol, Keck Sch Med, Los Angeles, CA 90033 USA
dc.description.affiliationUniv So Calif, Keck Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
dc.description.affiliationCALTECH, Pasadena, CA 91125 USA
dc.description.affiliationUniv Calif Santa Barbara, Ctr Stem Cell Biol & Engn, Santa Barbara, CA 93106 USA
dc.description.affiliationPeking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
dc.description.affiliationChang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
dc.description.affiliationHosp Evangel Curitiba, Curitiba, Parana, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Retina Sect, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Retina Sect, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCIRM
dc.description.sponsorshipResearch to Prevent Blindness and NEI
dc.description.sponsorshipIDCIRM: DRI-01444
dc.description.sponsorshipIDResearch to Prevent Blindness and NEI: EY03040
dc.format.extent186-191
dc.identifierhttp://dx.doi.org/10.1159/000338749
dc.identifier.citationOphthalmic Research. Basel: Karger, v. 48, n. 4, p. 186-191, 2012.
dc.identifier.doi10.1159/000338749
dc.identifier.issn0030-3747
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34386
dc.identifier.wosWOS:000307874500004
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofOphthalmic Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectTransplantationen
dc.subjectStem cellsen
dc.subjectParyleneen
dc.subjectRetinal pigment epitheliumen
dc.subjectRoyal College of Surgeon ratsen
dc.titleA Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayeren
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral