The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome

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dc.contributor.authorCamargo, A. A.
dc.contributor.authorSamaia, HPB
dc.contributor.authorDias-Neto, E.
dc.contributor.authorSimao, D. F.
dc.contributor.authorMigotto, I. A.
dc.contributor.authorBriones, Marcelo Ribeiro da Silva [UNIFESP]
dc.contributor.authorCosta, F. F.
dc.contributor.authorNagai, M. A.
dc.contributor.authorVerjovski-Almeida, S.
dc.contributor.authorZago, M. A.
dc.contributor.authorAndrade, Luiz Eduardo Coelho [UNIFESP]
dc.contributor.authorCarrer, H.
dc.contributor.authorEl-Dorry, HFA
dc.contributor.authorEspreafico, E. M.
dc.contributor.authorHabr-Gama, A.
dc.contributor.authorGiannella-Neto, D.
dc.contributor.authorGoldman, G. H.
dc.contributor.authorGruber, A.
dc.contributor.authorHackel, C.
dc.contributor.authorKimura, Edna Teruko [UNIFESP]
dc.contributor.authorMaciel, Rui Monteiro de Barros [UNIFESP]
dc.contributor.authorMarie, SKN
dc.contributor.authorMartins, EAL
dc.contributor.authorNobrega, M. P.
dc.contributor.authorPaco-Larson, M. L.
dc.contributor.authorPardini, MIMC
dc.contributor.authorPereira, G. G.
dc.contributor.authorPesquero, João Bosco [UNIFESP]
dc.contributor.authorRodrigues, V
dc.contributor.authorRogatto, SR
dc.contributor.authorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.authorSogayar, M. C.
dc.contributor.authorSonati, MDF
dc.contributor.authorTajara, E. H.
dc.contributor.authorValentini, Sandro Roberto
dc.contributor.authorAlberto, F. L.
dc.contributor.authorAmaral, MEJ
dc.contributor.authorAneas, I
dc.contributor.authorArnaldi, Liliane Aparecida Teixeira [UNIFESP]
dc.contributor.authorAssis, A. M. de
dc.contributor.authorBengtson, M. H.
dc.contributor.authorBergamo, N. A.
dc.contributor.authorBombonato, V
dc.contributor.authorCamargo, MER de
dc.contributor.authorCanevari, R. A.
dc.contributor.authorCarraro, D. M.
dc.contributor.authorCerutti, Janete Maria [UNIFESP]
dc.contributor.authorCorrea, MLC
dc.contributor.authorCorrea, RFR
dc.contributor.authorCosta, MCR
dc.contributor.authorCurcio, C.
dc.contributor.authorHokama, POM
dc.contributor.authorFerreira, AJS
dc.contributor.authorFuruzawa, Gilberto Koiti [UNIFESP]
dc.contributor.authorGushiken, T.
dc.contributor.authorHo, P. L.
dc.contributor.authorKimura, E.
dc.contributor.authorKrieger, J. E.
dc.contributor.authorLeite, LCC
dc.contributor.authorMajumder, P.
dc.contributor.authorMarins, M.
dc.contributor.authorMarques, E. R.
dc.contributor.authorMelo, Analy Salles de Azevedo [UNIFESP]
dc.contributor.authorMelo, M.
dc.contributor.authorMestriner, Carlos Alberto
dc.contributor.authorMiracca, E. C.
dc.contributor.authorMiranda, D. C.
dc.contributor.authorNascimento, Ana Lucia Tabet Oller do
dc.contributor.authorNobrega, F. G.
dc.contributor.authorOjopi, EPB
dc.contributor.authorPandolfi, JRC
dc.contributor.authorPessoa, Luciana Gilbert [UNIFESP]
dc.contributor.authorPrevedel, A. C.
dc.contributor.authorRahal, P.
dc.contributor.authorRainho, C. A.
dc.contributor.authorReis, EMR
dc.contributor.authorRibeiro, M. L.
dc.contributor.authorRos, N. da
dc.contributor.authorSa, R. G. de
dc.contributor.authorSales, M. M.
dc.contributor.authorSant'anna, S. C.
dc.contributor.authorSantos, M. L. dos
dc.contributor.authorSilva, A. M. da
dc.contributor.authorSilva, Neusa Pereira da [UNIFESP]
dc.contributor.authorSilva, W. A.
dc.contributor.authorSilveira, R. A. da
dc.contributor.authorSousa, J. F.
dc.contributor.authorStecconi, D.
dc.contributor.authorTsukumo, F.
dc.contributor.authorValente, V
dc.contributor.authorSoares, F.
dc.contributor.authorMoreira, E. S.
dc.contributor.authorNunes, D. N.
dc.contributor.authorCorrea, R. G.
dc.contributor.authorZalcberg, H.
dc.contributor.authorCarvalho, A. F.
dc.contributor.authorReis, LFL
dc.contributor.authorBrentani, Ricardo Renzo [UNIFESP]
dc.contributor.authorSimpson, AJG
dc.contributor.authorSouza, S. J. de
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFac Med Ribeirao Preto
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionHosp AC Camargo Fund Antonio Prudente
dc.date.accessioned2016-01-24T12:31:30Z
dc.date.available2016-01-24T12:31:30Z
dc.date.issued2001-10-09
dc.description.abstractopen reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. in our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. in this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. the distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. the experimental joining of the scaffold components, by reverse transcription-PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning.en
dc.description.affiliationLudwig Inst Canc Res, BR-01509010 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Reumatol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Biofis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Hemoctr, BR-13089970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Radiol, BR-01296903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05513970 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Clin Med, BR-14049900 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Pathogenicos, BR-14049900 São Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13418900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Inst Coracao, INCOR, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Lab Nutr & Doencas Metab, BR-01246 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Neurol, BR-01246 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecn, Dept Patol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciencias Med, Dept Med Genet, BR-13081970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Histol Embriol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, BR-04029032 São Paulo, Brazil
dc.description.affiliationInst Butantan, Ctr Biotecnol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Hemoctr, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciencias Med, Inst Biol, Dept Genet & Evolucao, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618000 São Paulo, Brazil
dc.description.affiliationEscola Paulista Med, Dept Ginecol & Obstet, BR-04301900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut & Araraquara, Dept Ciencias Biol, BR-14801902 São Paulo, Brazil
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, Dept Anat Patol, BR-01509010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Reumatol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Biofis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, BR-04029032 São Paulo, Brazil
dc.description.affiliationUnifespEscola Paulista Med, Dept Ginecol & Obstet, BR-04301900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent12103-12108
dc.identifierhttp://dx.doi.org/10.1073/pnas.201182798
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 98, n. 21, p. 12103-12108, 2001.
dc.identifier.doi10.1073/pnas.201182798
dc.identifier.issn0027-8424
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26642
dc.identifier.wosWOS:000171558900052
dc.language.isoeng
dc.publisherNatl Acad Sciences
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe contribution of 700,000 ORF sequence tags to the definition of the human transcriptomeen
dc.typeinfo:eu-repo/semantics/article
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