Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats

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dc.contributor.authorMaganhin, Carla C. [UNIFESP]
dc.contributor.authorSimoes, Ricardo S.
dc.contributor.authorFuchs, Luiz F. P.
dc.contributor.authorSasso, Gisela R. S. [UNIFESP]
dc.contributor.authorSimoes, Manuel J. [UNIFESP]
dc.contributor.authorBaracat, Edmund C.
dc.contributor.authorSoares, Jose M.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:37:32Z
dc.date.available2016-01-24T14:37:32Z
dc.date.issued2014-07-01
dc.description.abstractObjective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats.Design: Experimental research.Setting: University research laboratory.Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary.Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis.Result(s): the changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups.Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine.en
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, São Paulo Sch Med, Dept Obstet & Gynecol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Genet Paulista Sch Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Genet Paulista Sch Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/60412-7
dc.description.sponsorshipIDFAPESP: 2007/54398-4
dc.format.extent291-298
dc.identifierhttp://dx.doi.org/10.1016/j.fertnstert.2014.04.006
dc.identifier.citationFertility and Sterility. New York: Elsevier B.V., v. 102, n. 1, p. 291-298, 2014.
dc.identifier.doi10.1016/j.fertnstert.2014.04.006
dc.identifier.issn0015-0282
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37935
dc.identifier.wosWOS:000338932300049
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFertility and Sterility
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectGene expressionen
dc.subjectmelatoninen
dc.subjectovaryen
dc.subjectraten
dc.subjectsteroidogenesisen
dc.titleMelatonin influences on steroidogenic gene expression in the ovary of pinealectomized ratsen
dc.typeArtigo
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