The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines

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dc.contributor.authorSouza, J. P. de
dc.contributor.authorCorrêa, Zélia Maria da Silva [UNIFESP]
dc.contributor.authorMarshall, J. C.
dc.contributor.authorAnteka, E.
dc.contributor.authorCoutinho, A. B.
dc.contributor.authorMartins, M. C.
dc.contributor.authorBurnier, M. N.
dc.contributor.institutionMcGill Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionRetina & Oncol Serv
dc.date.accessioned2016-01-24T12:41:07Z
dc.date.available2016-01-24T12:41:07Z
dc.date.issued2006-05-01
dc.description.abstractPurpose To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb) cell lines.Methods Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by imunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. the averaged results per condition were recorded. the Student's t-test was used to compare results from the cells cultured with and without amfenac.Result the Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. the two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immucytochemical analysis.Conclusion the selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.en
dc.description.affiliationMcGill Univ, Henry C Witelson Ocular Pathol Lab, Montreal, PQ, Canada
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, SP, Brazil
dc.description.affiliationRetina & Oncol Serv, Dept Ophthalmol, Porto Alegre, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent598-601
dc.identifierhttp://dx.doi.org/10.1038/sj.eye.6701938
dc.identifier.citationEye. London: Nature Publishing Group, v. 20, n. 5, p. 598-601, 2006.
dc.identifier.doi10.1038/sj.eye.6701938
dc.identifier.issn0950-222X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28853
dc.identifier.wosWOS:000237360000016
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofEye
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCOX-2en
dc.subjectretinoblastomaen
dc.subjectnepafenacen
dc.subjectcell linesen
dc.subjectimmunocytochemistryen
dc.titleThe effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell linesen
dc.typeinfo:eu-repo/semantics/article
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