BINDING OF HEPARIN AND COMPOUND-Y TO ENDOTHELIAL-CELLS STIMULATES THE SYNTHESIS OF AN ANTITHROMBOTIC HEPARAN-SULFATE PROTEOGLYCAN

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dc.contributor.authorPinhal, Maria Aparecida da Silva [UNIFESP]
dc.contributor.authorSilva, I. F.
dc.contributor.authorLee, T. C.
dc.contributor.authorDietrich, Carl Peter [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionGENELABS INC
dc.date.accessioned2018-06-15T14:28:09Z
dc.date.available2018-06-15T14:28:09Z
dc.date.issued1994-09-01
dc.description.abstractThe mechanism by which heparin and antithrombotic agents, including a cyclic octaphenolsulfonic acid (compound Y), stimulate the synthesis of an antithrombotic heparan sulfate by endothelial cells in culture was investigated. Compound Y increases the amount of heparan sulfate from the cell surface and secreted to the medium by endothelial cells by three-fold. Binding experiments have shown saturation of the endothelial cell receptors at a concentration of 0.16 mu M for heparin and 2.7 mu M for compound Y. The kinetic binding constants (Ks) for compound Y and heparin were 1,333 nM and 42 nM, respectively. It was also shown that both compounds bind to the same receptors. The Scatchard plots indicated that 1,319 nmoles compound Y and 35 nmoles heparin bound per microgram cell protein, indicating that 40-fold more molecules of compound Y bound to the receptors when compared to heparin. No significant internalization Of the compounds was observed.en
dc.description.affiliationESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04044020 SAO PAULO,BRAZIL
dc.description.affiliationGENELABS INC,REDWOOD CITY,CA 94063
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04044020 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent2191-2195
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 9, p. 2191-2195, 1994.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43077
dc.identifier.wosWOS:A1994PG03900013
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectENDOTHELIAL HEPARAN SULFATE AND HEPARINen
dc.subjectENDOTHELIAL HEPARAN SULFATE AND ANTITHROMBOTIC DRUGSen
dc.subjectENDOTHELIAL CELLSen
dc.subjectHEPARIN BINDINGen
dc.titleBINDING OF HEPARIN AND COMPOUND-Y TO ENDOTHELIAL-CELLS STIMULATES THE SYNTHESIS OF AN ANTITHROMBOTIC HEPARAN-SULFATE PROTEOGLYCANen
dc.typeinfo:eu-repo/semantics/article
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