Co-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitro

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dc.contributor.authorPopi, Ana F. [UNIFESP]
dc.contributor.authorMotta, Fabiana L. T. [UNIFESP]
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.authorLopes, Jose D. [UNIFESP]
dc.contributor.authorMariano, Mario [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:51:59Z
dc.date.available2016-01-24T13:51:59Z
dc.date.issued2009-01-01
dc.description.abstractWe previously demonstrated that B-1b cells can undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to substrate in vitro. Here we followed the expression of surface markers and transcription factors during this differentiation. B-1b cells spontaneously express both myeloid and lymphoid restricted transcription factors. When induced to differentiate into a phagocyte, the lymphoid genes E box protein (E2A), early B-cell factor (EBF), paired box 5 (Pax5) are down-modulated, while expression of genes related to myeloid commitment is sustained. Furthermore, B-1b cell-derived phagocytes (B-1CDPs) decrease immunoglobulin M (IgM) expression but retain the expression of the heavy chain variable gene VH11 or VH12, an immunoglobulin gene rearrangement predominantly expressed by B-1 cells. the maintenance of lymphoid characteristics in B-1CDPs characterizes a unique type of phagocyte, not related to monocyte-derived macrophages.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, CEDEME Ctr Desenvolvimento Modelos Expt Med & Bio, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, CEDEME Ctr Desenvolvimento Modelos Expt Med & Bio, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 04/14837-0
dc.description.sponsorshipIDFAPESP: 04/08506-1
dc.format.extent114-122
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2567.2008.02883.x
dc.identifier.citationImmunology. Malden: Wiley-Blackwell Publishing, Inc, v. 126, n. 1, p. 114-122, 2009.
dc.identifier.doi10.1111/j.1365-2567.2008.02883.x
dc.identifier.issn0019-2805
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31110
dc.identifier.wosWOS:000261528300013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofImmunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectB-1 cellsen
dc.subjectdifferentiationen
dc.subjectmacrophageen
dc.subjectphagocytosisen
dc.subjecttranscription factorsen
dc.titleCo-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitroen
dc.typeinfo:eu-repo/semantics/article
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