Enhancement of the citrulline-nitric oxide cycle in astroglioma cells by the proline-rich peptide-10c from Bothrops jararaca venom

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dc.contributor.authorOliveira, Eduardo Fontana de [UNIFESP]
dc.contributor.authorGuerreiro, Juliano R.
dc.contributor.authorSilva, Carlos A.
dc.contributor.authorSouza Benedetti, Gabriel Fernando de [UNIFESP]
dc.contributor.authorLebrun, Ivo
dc.contributor.authorUlrich, Henning
dc.contributor.authorLameu, Claudiana
dc.contributor.authorCamargo, Antonio C. M.
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2016-01-24T14:05:48Z
dc.date.available2016-01-24T14:05:48Z
dc.date.issued2010-12-06
dc.description.abstractThe biological activity of the proline rich decapeptde Bj PRO 10c a processing product of the C type natriuretic peptide precursor protein, expressed in the brain and the venom gland of the pit viper Bothrops jararaca, was originally attributed to the inhibition of the somatic angiotensm converting enzyme activity with subsequent ant hypertensive effect However recent results suggest broader biological activity may also be involved in the cardiovascular effects of this peptide Here we show that Bj PRO 10c enhances and sustains the generation of nitric made (NO) by regulating argininosuccinate synthase activity and thereby velocity of the citrulline NO cycle Bj PRO 10c-mediated effects not restricted to the cardiovascular system since NO production was also induced in cells of astroglial origin Bj PRO 10c was internalized by C6 astroglioma cells where it induces NO production and upregulation of the citrulline NO cycle cells in a dose dependent fashion in view of that, astroglial cells function as L arginine pool for NO production in neighboring neurons, we suggest a regulatory function for Bj PRO-10c on the metabolism of this gaseous neurotransmitter in the CNS Moreover, proliferation of astroglial cells was reduced in the presence of Bj PRO 10c however, cell death was not induced Since NO donors have been studied for the treatment of solid cancers Bj PRO 10c may serve as structural model for developing drugs to improve the effects of cancer therapy based on the peptide's ability to augment NO production (C) 2010 Elsevier B V All rights reserveden
dc.description.affiliationInst Butantan, Ctr Appl Toxinol CAT CEPID, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, SP, Brazil
dc.description.affiliationESALQ USP, Lab Bioquim Prot, Ctr Biotecnol Agr CEBTEC, Dept Ciencias Biol, São Paulo, SP, Brazil
dc.description.affiliationUniv Fed Abc, Nat & Human Sci Ctr, Santo Andre, Brazil
dc.description.affiliationInst Butantan, Lab Bioquim & Biofis, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim, Inst Quim, BR-05508 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent11-19
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2010.09.067
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 1363, p. 11-19, 2010.
dc.identifier.doi10.1016/j.brainres.2010.09.067
dc.identifier.issn0006-8993
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33164
dc.identifier.wosWOS:000284789400002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectArgininosuccinate synthaseen
dc.subjectBothrops jararacaen
dc.subjectC6 astroglioma cellen
dc.subjectNitric oxideen
dc.subjectProline rich peptideen
dc.titleEnhancement of the citrulline-nitric oxide cycle in astroglioma cells by the proline-rich peptide-10c from Bothrops jararaca venomen
dc.typeinfo:eu-repo/semantics/article
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