TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction

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dc.contributor.authorAndrade-Oliveira, Vinicius [UNIFESP]
dc.contributor.authorCampos, Erika F. [UNIFESP]
dc.contributor.authorGoncalves-Primo, Amador
dc.contributor.authorGrenzi, Patricia C. [UNIFESP]
dc.contributor.authorMedina-Pestana, Jose O. [UNIFESP]
dc.contributor.authorTedesco-Silva, Helio
dc.contributor.authorGerbase-DeLima, Maria [UNIFESP]
dc.contributor.institutionAssoc Fundo Incent Pesquisa
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHosp Rim & Hipertensao
dc.date.accessioned2016-01-24T14:27:43Z
dc.date.available2016-01-24T14:27:43Z
dc.date.issued2012-09-27
dc.description.abstractBackground. the participation of Toll-like receptor (TLR) 4, an innate immunity receptor, has been previously demonstrated in the pathogenesis of acute renal injury. We aimed to investigate whether messenger RNA (mRNA) levels of TLR4 and its adapter molecule, myeloid differentiation primary response gene (MYD) 88, are associated with delayed graft function (DGF) and could be used as biomarkers of its occurrence.Methods. TLR4 and MYD88 gene mRNA levels were evaluated with real-time polymerase chain reaction, in preimplantation biopsies (n=89) and first day posttransplantation samples of urine (n=67) and blood (n=80) from graft recipients and analyzed according to donor type (living or deceased) and DGF occurrence.Results. Expression levels of both genes were higher in biopsies from deceased donors than from living donors (P<0.001 for both) but did not differ between deceased-donor kidney transplants with and without DGF; in urine, TLR4 expression levels were higher in patients with prolonged DGF (DGF lasting 914 days) (P=0.05, compared with cases without DGF); in blood, lower mRNA levels of TLR4 and MYD88 predicted pDGF occurrence with an accuracy of 86% and 87%, respectively.Conclusion. the expression levels of TLR4 and MYD88 were higher in kidneys from deceased donors than from living donors. Lower levels of expression of both genes in blood were associated with DGF occurrence. the prediction of prolonged DGF by low TLR4 and MYD88 expression levels in blood with a greater the 85% accuracy was the most important finding of this study.en
dc.description.affiliationAssoc Fundo Incent Pesquisa, Inst Imunogenet, BR-04040031 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationHosp Rim & Hipertensao, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipAssociacao Fundo de Incentivo a Pesquisa
dc.description.sponsorshipIDFAPESP: 2008/51327-1
dc.format.extent589-595
dc.identifierhttp://dx.doi.org/10.1097/TP.0b013e31825db680
dc.identifier.citationTransplantation. Philadelphia: Lippincott Williams & Wilkins, v. 94, n. 6, p. 589-595, 2012.
dc.identifier.doi10.1097/TP.0b013e31825db680
dc.identifier.issn0041-1337
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35287
dc.identifier.wosWOS:000309223100012
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofTransplantation
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDelayed graft functionen
dc.subjectTLR4en
dc.subjectInnate immunityen
dc.subjectKidney/renal transplantationen
dc.subjectIschemia-reperfusionen
dc.titleTLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunctionen
dc.typeinfo:eu-repo/semantics/article
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