Bovine papillomavirus transmission and chromosomal aberrations: an experimental model

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dc.contributor.authorStocco, Rita de Cassia
dc.contributor.authorLindsey, Charles Julian [UNIFESP]
dc.contributor.authorFerraz, Oilita Pereira
dc.contributor.authorPinto, José Ricardo
dc.contributor.authorMirandola, Regina S.
dc.contributor.authorBenesi, Fernando J.
dc.contributor.authorBirgel, Eduardo H.
dc.contributor.authorPereira, Carlos Alberto de Bragança
dc.contributor.authorBeçak, Willy
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-06-15T18:02:16Z
dc.date.available2018-06-15T18:02:16Z
dc.date.issued1998-09-01
dc.description.abstractEnzootic haematuria and urinary bladder cancer in cattle are associated with feeding on bracken fern and bovine papillomavirus (BPV) infection. An increased rate of chromosomal aberrations in peripheral blood lymphocytes from chronically affected haematuric cows raised in bracken fern pastures has been reported, suggesting the presence of BPV in the peripheral blood of afflicted animals. The purpose of the present investigation was to examine the role of peripheral blood as a potential BPV-transmitting agent and search for clastogenic effects in experimentally infected animals kept on a bracken fern-free diet. Healthy cows were inoculated with blood samples of haematuric animals every two weeks for 18 months. Recipient cows, their offspring, donor animals and a control group were kept on a bracken fern-free diet throughout the experiment. Clinical and molecular analyses for detection of BPV infection were carried out periodically in all groups, Short-term lymphocyte cultures were performed to assess chromosomal aberration levels. The donor cows, the recipient cows and their offspring presented increased levels of chromosomal aberrations. BPV-2 DNA was identified by Southern blotting, PCR and cycle-sequencing of PCR products in peripheral blood of donor and recipient animals and in the progeny of recipient animals. Data support both the concept that BPV can be transmitted through blood and the hypothesis that infection with the virus causes the clastogenic alterations observed in the present experimental model. The presence of BPV-2 DNA and chromosomal alterations in peripheral blood of offspring at the moment of birth is evidence for vertical transmission of BPV.en
dc.description.affiliationInst Butantan, Genet Lab, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationInst Butantan, Fazenda Sao Joaquim, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Biofis, BR-04041990 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med Vet & Zootecn, Med Clin, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Matemat & Estatist, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Biofis, BR-04041990 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent2127-2135
dc.identifierhttps://dx.doi.org/10.1099/0022-1317-79-9-2127
dc.identifier.citationJournal Of General Virology. Reading: Soc General Microbiology, v. 79, n. 9, p. 2127-2135, 1998.
dc.identifier.doi10.1099/0022-1317-79-9-2127
dc.identifier.issn0022-1317
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/44371
dc.identifier.wosWOS:000075592600007
dc.language.isoeng
dc.publisherSoc General Microbiology
dc.relation.ispartofJournal Of General Virology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleBovine papillomavirus transmission and chromosomal aberrations: an experimental modelen
dc.typeinfo:eu-repo/semantics/article
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