A Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytes

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dc.contributor.authorPires Goncalves, Leticia Christina
dc.contributor.authorTonelli, Renata Rosito [UNIFESP]
dc.contributor.authorBagnaresi, Piero [UNIFESP]
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.authorFerreira, Antonio Gilberto
dc.contributor.authorBastos, Erick Leite
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2016-01-24T14:31:08Z
dc.date.available2016-01-24T14:31:08Z
dc.date.issued2013-01-16
dc.description.abstractA model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its photophysical properties are not notably pH-sensitive. Fluorescence imaging microscopy of erythrocytes infected with Plasmodium falciparum, a causative agent of malaria in humans, showed that only the parasite was stained. Z-stacking analysis suggested that the probe accumulates proximal to the nucleus of the parasite. Indicaxanthin, one of the natural fluorescent betalains found in the petals of certain flowers, did not stain the parasite or the red blood cell.en
dc.description.affiliationUniv Fed ABC, Ctr Ciencias Nat & Humanas, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Ciencias Biol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunobiol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Quim, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Quim Fundamental, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunobiol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 07/00684-6
dc.description.sponsorshipIDFAPESP: 10/15042-2
dc.description.sponsorshipIDFAPESP: 11/23036-5
dc.description.sponsorshipIDFAPESP: 07/59407-1
dc.description.sponsorshipIDCoordination for the Improvement of Higher Education Personnel: PNPD 427-10/2009
dc.format.extent7
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0053874
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 8, n. 1, 7 p., 2013.
dc.identifier.doi10.1371/journal.pone.0053874
dc.identifier.fileWOS000313682700060.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35881
dc.identifier.wosWOS:000313682700060
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytesen
dc.typeinfo:eu-repo/semantics/article
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