Molecular characterization and intracellular distribution of the alpha 5 subunit of Trypanosoma cruzi 20S proteasome

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dc.contributor.authorGutierrez, Bessy
dc.contributor.authorOsorio, Luis
dc.contributor.authorMotta, Maria Cristina M.
dc.contributor.authorHuima-Byron, Telervo
dc.contributor.authorErdjument-Bromage, Heydeie
dc.contributor.authorMunoz, Christian
dc.contributor.authorSagua, Hernan
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorEcheverria, Alex
dc.contributor.authorAraya, Jorge E.
dc.contributor.authorGonzalez, Jorge
dc.contributor.institutionUniv Antofagasta
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionNew York Blood Ctr
dc.contributor.institutionMem Sloan Kettering Canc Ctr
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionN Catholic Univ
dc.date.accessioned2016-01-24T13:58:58Z
dc.date.available2016-01-24T13:58:58Z
dc.date.issued2009-12-01
dc.description.abstractThree different monoclonal antibodies were produced against Trypanosona cruzi proteasomes. These antibodies were shown to react with a single 27-kDa hand on immunoblots of purified proteasomes. Using a 7E5 monoclonal antibody (IgG1) that recognized the alpha 5 subunit of protozoan protease we have studied the intracellular distribution of the T cruzi 20S proteasome. Contrary to all cell types described to date, T cruzi 20S proteasome was found not only in the cytoplasm and nucleus but also in the kinetoplast. As revealed by confocal microscopy, the reactivity of monoclonal antibody 7E5 was highly specific for protozoan proteasome because the antibody recognized only the proteasomes from parasites and not those from the mammalian host in T. cruzi infected cells. These findings were confirmed by immunoblots or immunoprecipitations, followed by chymotrypsin-like activity detection in kinetoplasts isolated by differential centrifugation and sucrose density gradients. Proteasome 20S was present in all T cruzi stages and only slight differences in terms of relative abundance were found. the potential role of the proteasome in kinetoplast remodeling remains to be determined. (C) 2009 Published by Elsevier Ireland Ltd.en
dc.description.affiliationUniv Antofagasta, Fac Hlth Sci, Mol Parasitol Unit, Antofagasta, Chile
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, Brazil
dc.description.affiliationNew York Blood Ctr, New York, NY 10021 USA
dc.description.affiliationMem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA
dc.description.affiliationEscola Paulista Med UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationN Catholic Univ, Ctr Biotechnol, Antofagasta, Chile
dc.description.affiliationUnifespEscola Paulista Med UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent367-374
dc.identifierhttp://dx.doi.org/10.1016/j.parint.2009.07.012
dc.identifier.citationParasitology International. Clare: Elsevier B.V., v. 58, n. 4, p. 367-374, 2009.
dc.identifier.doi10.1016/j.parint.2009.07.012
dc.identifier.issn1383-5769
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31995
dc.identifier.wosWOS:000271496300009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofParasitology International
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectAntibodiesen
dc.subjectProteasomeen
dc.subjectIntracellular localizationen
dc.titleMolecular characterization and intracellular distribution of the alpha 5 subunit of Trypanosoma cruzi 20S proteasomeen
dc.typeinfo:eu-repo/semantics/article
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