Molecular characterization and intracellular distribution of the alpha 5 subunit of Trypanosoma cruzi 20S proteasome
dc.contributor.author | Gutierrez, Bessy | |
dc.contributor.author | Osorio, Luis | |
dc.contributor.author | Motta, Maria Cristina M. | |
dc.contributor.author | Huima-Byron, Telervo | |
dc.contributor.author | Erdjument-Bromage, Heydeie | |
dc.contributor.author | Munoz, Christian | |
dc.contributor.author | Sagua, Hernan | |
dc.contributor.author | Mortara, Renato A. [UNIFESP] | |
dc.contributor.author | Echeverria, Alex | |
dc.contributor.author | Araya, Jorge E. | |
dc.contributor.author | Gonzalez, Jorge | |
dc.contributor.institution | Univ Antofagasta | |
dc.contributor.institution | Universidade Federal do Rio de Janeiro (UFRJ) | |
dc.contributor.institution | New York Blood Ctr | |
dc.contributor.institution | Mem Sloan Kettering Canc Ctr | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor.institution | N Catholic Univ | |
dc.date.accessioned | 2016-01-24T13:58:58Z | |
dc.date.available | 2016-01-24T13:58:58Z | |
dc.date.issued | 2009-12-01 | |
dc.description.abstract | Three different monoclonal antibodies were produced against Trypanosona cruzi proteasomes. These antibodies were shown to react with a single 27-kDa hand on immunoblots of purified proteasomes. Using a 7E5 monoclonal antibody (IgG1) that recognized the alpha 5 subunit of protozoan protease we have studied the intracellular distribution of the T cruzi 20S proteasome. Contrary to all cell types described to date, T cruzi 20S proteasome was found not only in the cytoplasm and nucleus but also in the kinetoplast. As revealed by confocal microscopy, the reactivity of monoclonal antibody 7E5 was highly specific for protozoan proteasome because the antibody recognized only the proteasomes from parasites and not those from the mammalian host in T. cruzi infected cells. These findings were confirmed by immunoblots or immunoprecipitations, followed by chymotrypsin-like activity detection in kinetoplasts isolated by differential centrifugation and sucrose density gradients. Proteasome 20S was present in all T cruzi stages and only slight differences in terms of relative abundance were found. the potential role of the proteasome in kinetoplast remodeling remains to be determined. (C) 2009 Published by Elsevier Ireland Ltd. | en |
dc.description.affiliation | Univ Antofagasta, Fac Hlth Sci, Mol Parasitol Unit, Antofagasta, Chile | |
dc.description.affiliation | Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, Brazil | |
dc.description.affiliation | New York Blood Ctr, New York, NY 10021 USA | |
dc.description.affiliation | Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA | |
dc.description.affiliation | Escola Paulista Med UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil | |
dc.description.affiliation | N Catholic Univ, Ctr Biotechnol, Antofagasta, Chile | |
dc.description.affiliationUnifesp | Escola Paulista Med UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil | |
dc.description.source | Web of Science | |
dc.format.extent | 367-374 | |
dc.identifier | http://dx.doi.org/10.1016/j.parint.2009.07.012 | |
dc.identifier.citation | Parasitology International. Clare: Elsevier B.V., v. 58, n. 4, p. 367-374, 2009. | |
dc.identifier.doi | 10.1016/j.parint.2009.07.012 | |
dc.identifier.issn | 1383-5769 | |
dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/31995 | |
dc.identifier.wos | WOS:000271496300009 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Parasitology International | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.subject | Trypanosoma cruzi | en |
dc.subject | Antibodies | en |
dc.subject | Proteasome | en |
dc.subject | Intracellular localization | en |
dc.title | Molecular characterization and intracellular distribution of the alpha 5 subunit of Trypanosoma cruzi 20S proteasome | en |
dc.type | info:eu-repo/semantics/article |