Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation

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dc.contributor.authorCarvalho, ACP
dc.contributor.authorSharpe, J.
dc.contributor.authorRosenstock, T. R.
dc.contributor.authorTeles, AFV
dc.contributor.authorYoule, R. J.
dc.contributor.authorSmaili, Soraya Soubhi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionNINDS
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:37:29Z
dc.date.available2016-01-24T12:37:29Z
dc.date.issued2004-12-01
dc.description.abstractIn the present study, we evaluated proapoptotic protein Bax on mitochondria and Ca2+ homeostasis in primary cultured astrocytes. We found that recombinant Bax (rBax, 10 and 100 ng/ml) induces a loss in mitochondrial membrane potential (DeltaPsi(m)). This effect might be related to the inhibition of respiratory rates and a partial release of cytochrome c, which may change mitochondrial morphology. the loss of DeltaPsi(m) and a selective permeabilization of mitochondrial membranes contribute to the release of Ca2+ from the mitochondria. This was inhibited by cyclosporin A (5 muM) and Ruthenium Red (1 mug/ml), indicating the involvement of mitochondrial Ca2+ transport mechanisms. Bax-induced mitochondrial Ca2+ release evokes Ca2+ waves and wave propagation between cells. Our results show that Bax induces mitochondrial alteration that affects Ca2+ homeostasis and signaling. These changes show that Ca2+ signals might be correlated with the proapoptotic activities of Bax.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, INFAR, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliationNINDS, Biochem Sect, NIH, Bethesda, MD 20892 USA
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, INFAR, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1265-1276
dc.identifierhttp://dx.doi.org/10.1038/sj.cdd.4401508
dc.identifier.citationCell Death and Differentiation. London: Nature Publishing Group, v. 11, n. 12, p. 1265-1276, 2004.
dc.identifier.doi10.1038/sj.cdd.4401508
dc.identifier.issn1350-9047
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28023
dc.identifier.wosWOS:000225109800004
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofCell Death and Differentiation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBaxen
dc.subjectcalcium signalingen
dc.subjectmitochondriaen
dc.subjectendoplasmic reticulumen
dc.subjectcalcium wavesen
dc.subjectwave propagationen
dc.subjectapoptosisen
dc.titleBax affects intracellular Ca2+ stores and induces Ca2+ wave propagationen
dc.typeinfo:eu-repo/semantics/article
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