The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms

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dc.contributor.authorOliveira, Sarah Almeida Coelho
dc.contributor.authorZambrana, Jéssica Rabelo Mina
dc.contributor.authorDi Iorio, Fernanda Bispo Reis
dc.contributor.authorPereira, Cristiane Aparecida
dc.contributor.authorJorge, Antonio Olavo Cardoso
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:52Z
dc.date.available2015-06-14T13:46:52Z
dc.date.issued2014-01-01
dc.description.abstractThe aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.en
dc.description.affiliationUniversidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral Diagnosis
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact Sciences
dc.description.affiliationUnifespUNIFESP, Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact Sciences
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 09/52048-1
dc.description.sponsorshipIDFAPESP: 2010/00879-4
dc.format.extent22-27
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242013005000024
dc.identifier.citationBrazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014.
dc.identifier.doi10.1590/S1806-83242013005000024
dc.identifier.fileS1806-83242014000100217.pdf
dc.identifier.issn1806-8324
dc.identifier.scieloS1806-83242014000100217
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8184
dc.identifier.wosWOS:000328723700004
dc.language.isoeng
dc.publisherSociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.relation.ispartofBrazilian Oral Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiofilmsen
dc.subjectCitrus limonumen
dc.subjectCitrus aurantiumen
dc.titleThe antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilmsen
dc.typeinfo:eu-repo/semantics/article
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