Heme oxygenase 1 improves glucoses metabolism and kidney histological alterations in diabetic rats

dc.contributor.authorPtilovanciv, Ellen O. N. [UNIFESP]
dc.contributor.authorFernandes, Gabryelle S. [UNIFESP]
dc.contributor.authorTeixeira, Luciana C. [UNIFESP]
dc.contributor.authorReis, Luciana A. [UNIFESP]
dc.contributor.authorPessoa, Edson A. [UNIFESP]
dc.contributor.authorConvento, Marcia B. [UNIFESP]
dc.contributor.authorSimoes, Manuel J. [UNIFESP]
dc.contributor.authorAlbertoni, Guilherme A. [UNIFESP]
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.authorBorges, Fernanda T. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:08Z
dc.date.available2016-01-24T14:31:08Z
dc.date.issued2013-01-16
dc.description.abstractOne important concern in the treatment of diabetes is the maintenance of glycemic levels and the prevention of diabetic nephropathy. Inducible heme oxygenase 1 (HO-1) is a rate-limiting enzyme thought to have antioxidant and cytoprotective roles. the goal of the present study was to analyze the effect of HO-1 induction in chronically hyperglycemic rats. the hyperglycemic rats were divided into two groups: one group, called STZ, was given a single injection of streptozotocin; and the other group was given a single streptozotocin injection as well as daily injections of hemin, an HO-1 inducer, over 60 days (STZ + HEME). A group of normoglycemic, untreated rats was used as the control (CTL).Body weight, diuresis, serum glucose levels, microalbuminuria, creatinine clearance rate, urea levels, sodium excretion, and lipid peroxidation were analyzed. Histological alterations and immunohistochemistry for HO-1 and inducible nitric oxide synthase (iNOS) were assessed. After 60 days, the STZ group exhibited an increase in blood glucose, diuresis, urea, microalbuminuria, and sodium excretion. There was no weight gain, and there was a decrease in creatinine clearance in comparison to the CTL group. in the STZ + HEME group there was an improvement in the metabolic parameters and kidney function, a decrease in blood glucose, serum urea, and microalbuminuria, and an increase of creatinine clearance, in comparison to the STZ group.There was glomerulosclerosis, collagen deposition in the STZ rats and increase in iNOS and HO-1 expression. in the STZ + HEME group, the glomerulosclerosis and fibrosis was prevented and there was an increase in the expression of HO-1, but decrease in iNOS expression and lipid peroxidation. in conclusion, our data suggest that chronic induction of HO-1 reduces hyperglycemia, improves glucose metabolism and, at least in part, protects the renal tissue from hyperglycemic injury, possibly through the antioxidant activity of HO-1.en
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Morphol Dept, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Morphol Dept, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1186/1758-5996-5-3
dc.identifier.citationDiabetology & Metabolic Syndrome. London: Biomed Central Ltd, v. 5, 8 p., 2013.
dc.identifier.doi10.1186/1758-5996-5-3
dc.identifier.fileWOS000314977200001.pdf
dc.identifier.issn1758-5996
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35880
dc.identifier.wosWOS:000314977200001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofDiabetology & Metabolic Syndrome
dc.rightsAcesso aberto
dc.subjectHeme oxygenase 1en
dc.subjectNitric oxide synthaseen
dc.subjectRenal functionen
dc.subjectDiabetic nephropathyen
dc.subjectGlucose metabolismen
dc.titleHeme oxygenase 1 improves glucoses metabolism and kidney histological alterations in diabetic ratsen
dc.typeArtigo
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