Oxidative origin of sperm DNA fragmentation in the adult varicocele

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dc.citation.volume46pt_BR
dc.contributor.authorJeremias, Jessica Timóteo
dc.contributor.authorBelardin, Larissa Berloffa
dc.contributor.authorOkada, Fatima Kazue
dc.contributor.authorAntoniassi, Mariana Pereira
dc.contributor.authorFraietta, Renato
dc.contributor.authorBertolla, Ricardo Pimenta
dc.contributor.authorIntasqui, Paula
dc.contributor.authorLatteshttp://lattes.cnpq.br/5188240479960852pt_BR
dc.date.accessioned2021-01-15T11:47:59Z
dc.date.available2021-01-15T11:47:59Z
dc.date.issued2020-09-10
dc.description.abstractPurpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. Materials and Methods: A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student’s t test or a Mann-Whitney test. Results: The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidativeinduced DNA fragmentation. Conclusions: Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentAhead of printpt_BR
dc.identifierhttps://www.intbrazjurol.com.br/pdf/vol47n02/IBJU20190827.pdfpt_BR
dc.identifier.doi10.1590/S1677-5538.IBJU.2019.0827pt_BR
dc.identifier.issn1677-6119pt_BR
dc.identifier.issn1677-5538pt_BR
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/59037
dc.languageengpt_BR
dc.publisherBrazilian Society of Urology– SBUpt_BR
dc.relation.ispartofInternational Brazilian Journal of Urologypt_BR
dc.rightsinfo:eu-repo/semantics/openAccesspt_BR
dc.subjectDNA Fragmentationpt_BR
dc.subjectInfertility, Malept_BR
dc.subjectOxidative Stresspt_BR
dc.subjectVaricocelept_BR
dc.titleOxidative origin of sperm DNA fragmentation in the adult varicocelept_BR
dc.typeinfo:eu-repo/semantics/articlept_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoCirurgiapt_BR
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