Analysis of Genes Encoding Penicillin-Binding Proteins in Clinical Isolates of Acinetobacter baumannii

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dc.contributor.authorCayƓ, Rodrigo [UNIFESP]
dc.contributor.authorRodriguez, Maria-Cruz
dc.contributor.authorEspinal, Paula
dc.contributor.authorFernandez-Cuenca, Felipe
dc.contributor.authorOcampo-Sosa, Alain A.
dc.contributor.authorPascual, Alvaro
dc.contributor.authorAyala, Juan A.
dc.contributor.authorVila, Jordi
dc.contributor.authorMartinez-Martinez, Luis
dc.contributor.institutionUniv Hosp Marques de Valdecilla IFIMAV
dc.contributor.institutionUniv Cantabria
dc.contributor.institutionUniv Barcelona
dc.contributor.institutionUniv Hosp Virgen Macarena
dc.contributor.institutionUAM
dc.contributor.institutionUniversidade Federal de SĆ£o Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:29Z
dc.date.available2016-01-24T14:17:29Z
dc.date.issued2011-12-01
dc.description.abstractThere is limited information on the role of penicillin-binding proteins (PBPs) in the resistance of Acinetobacter baumannii to beta-lactams. This study presents an analysis of the allelic variations of PBP genes in A. baumannii isolates. Twenty-six A. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in Spain were included. the antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. Based on the six complete genomes of A. baumannii, the PBP genes were identified, and primers were designed for each gene. the nucleotide sequences of the genes identified that encode PBPs and the corresponding amino acid sequences were compared with those of ATCC 17978. Seven PBP genes and one monofunctional transglycosylase (MGT) gene were identified in the six genomes, encoding (i) four high-molecular-mass proteins (two of class A, PBP1a [ponA] and PBP1b [mrcB], and two of class B, PBP2 [pbpA or mrdA] and PBP3 [ftsI]), (ii) three low-molecular-mass proteins (two of type 5, PBP5/6 [dacC] and PBP6b [dacD], and one of type 7 (PBP7/8 [pbpG]), and (iii) a monofunctional enzyme (MtgA [mtgA]). Hot spot mutation regions were observed, although most of the allelic changes found translated into silent mutations. the amino acid consensus sequences corresponding to the PBP genes in the genomes and the clinical isolates were highly conserved. the changes found in amino acid sequences were associated with concrete clonal patterns but were not directly related to susceptibility or resistance to beta-lactams. An insertion sequence disrupting the gene encoding PBP6b was identified in an endemic carbapenem-resistant clone in one of the participant hospitals.en
dc.description.affiliationUniv Hosp Marques de Valdecilla IFIMAV, Microbiol Serv, Santander, Spain
dc.description.affiliationUniv Cantabria, Sch Med, Dept Mol Biol, Cantabria, Spain
dc.description.affiliationUniv Barcelona, Microbiol Serv, Hosp Clin, Sch Med, Barcelona, Spain
dc.description.affiliationUniv Hosp Virgen Macarena, Microbiol Serv, Seville, Spain
dc.description.affiliationUAM, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, Spain
dc.description.sourceWeb of Science
dc.description.sponsorshipInstituto de Salud Carlos III, Spanish Network for Research in Infectious Diseases (REIPI)
dc.description.sponsorshipFIS
dc.description.sponsorshipPDEE
dc.description.sponsorshipMICINN
dc.description.sponsorshipIDInstituto de Salud Carlos III, Spanish Network for Research in Infectious Diseases (REIPI): RD06/0008
dc.description.sponsorshipIDFIS: PI080209
dc.description.sponsorshipIDPDEE: protocol 4149/08-4
dc.description.sponsorshipIDMICINN: BFU2009-09200
dc.format.extent5907-5913
dc.identifierhttp://dx.doi.org/10.1128/AAC.00459-11
dc.identifier.citationAntimicrobial Agents and Chemotherapy. Washington: Amer Soc Microbiology, v. 55, n. 12, p. 5907-5913, 2011.
dc.identifier.doi10.1128/AAC.00459-11
dc.identifier.fileWOS000296920600065.pdf
dc.identifier.issn0066-4804
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34254
dc.identifier.wosWOS:000296920600065
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAnalysis of Genes Encoding Penicillin-Binding Proteins in Clinical Isolates of Acinetobacter baumanniien
dc.typeinfo:eu-repo/semantics/article
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